Construct: ORF TRCN0000469313
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010615.1_s317c1
- Derived from:
- ccsbBroadEn_11662
- DNA Barcode:
- GCGTCCTGTCCTACTGCTGGTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAMD4A (23034)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469313
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23034 | SAMD4A | sterile alpha motif domain ... | NM_001161576.2 | 38.3% | 37.5% | (many diffs) |
| 2 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449517.1 | 36.3% | 35.5% | (many diffs) |
| 3 | human | 23034 | SAMD4A | sterile alpha motif domain ... | NM_015589.6 | 33.9% | 33.2% | (many diffs) |
| 4 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449516.1 | 33.2% | 32.6% | (many diffs) |
| 5 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449514.1 | 32.2% | 31.6% | (many diffs) |
| 6 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449515.1 | 32.2% | 31.6% | (many diffs) |
| 7 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449519.1 | 21.1% | 20.4% | (many diffs) |
| 8 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449518.1 | 18.9% | 18.3% | (many diffs) |
| 9 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_028966.3 | 36.5% | 36.6% | (many diffs) |
| 10 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_001037221.2 | 32.1% | 32.4% | (many diffs) |
| 11 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | XM_006519627.2 | 30.2% | 30% | (many diffs) |
| 12 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_001163433.1 | 18.5% | 18.5% | (many diffs) |
| 13 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | XM_006519628.2 | 17.4% | 17.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gtttcgcgac caggtcgggg tgctggcggg ctggtttaag ggctggaacg 121 agtgcgagca gactgttgcg ctgctgtcgc tgctcaagcg cgtgagccag acccaggccc 181 gcttcctcca gctctgcctg gagcactcgc tggccgactg cgccgagctg cacgtcctcg 241 aacgcgaggc caacagcccc ggaatcatta accaatggca acaggaatcc aaggataaag 301 tgatttccct cctgttaact caTCTGCCTT TGCTGAAGCC AGGAAACCTC GACGCGAAAG 361 TAGAATATAT GAAACTGCTG CCCAAAACCC TGGCTCACTC TATTGAACAC AACCAGCACA 421 TTGAGGAGAG CAGGCAGCTG CTGTCCTATG CTTTGATACA TCCAGCCACT TCGTTAGAAG 481 ACCGTAGTGC TTTAGCCATG TGGCTGAATC ACTTGGAGGA CCGCACGTCG ACCAGCTTTG 541 GTGGCCAGAA CCGAGGCCGC TCAGACTCTG TGGATTATGG ACAGACACAC TACTATCACC 601 AAAGACAGAA CTCTGATGAC AAGCTCAATG GGTGGCAGAA CTCTCGGGAT TCTGGGATTT 661 GCATCAATGC CTCCAACTGG CAGGACAAAA GCATGGGGTG TGAGAATGGC CATGTGCCCC 721 TCTACTCCTC CTCATCTGTC CCCACCACAA TCAATACGAT TGGAACCAGC ACAAGTACAA 781 GTAAGTTCCC CGGAATCCCT TTAACGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCGTCCTG 961 TCCTACTGCT GGTGACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt