Construct: ORF TRCN0000469320
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001502.1_s317c1
- Derived from:
- ccsbBroadEn_11444
- DNA Barcode:
- TTCCGGTATACTCTACCGGGTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAALAD2 (10003)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469320
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | XM_017017046.2 | 71.2% | 70% | (many diffs) |
| 2 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | XR_001747711.2 | 52.1% | (many diffs) | |
| 3 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | XR_001747707.2 | 44.8% | (many diffs) | |
| 4 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | NM_001300930.2 | 43.5% | 42.3% | (many diffs) |
| 5 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | XM_017017044.2 | 41.5% | 40.4% | (many diffs) |
| 6 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | NM_005467.4 | 41.4% | 40.5% | (many diffs) |
| 7 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | XM_017017043.2 | 39.5% | 38.7% | (many diffs) |
| 8 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | XM_017017045.1 | 34.2% | 33.2% | (many diffs) |
| 9 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | XR_001747710.2 | 25.7% | (many diffs) | |
| 10 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | XR_001747709.2 | 25.7% | (many diffs) | |
| 11 | human | 10003 | NAALAD2 | N-acetylated alpha-linked a... | XR_001747708.2 | 22.6% | (many diffs) | |
| 12 | mouse | 72560 | Naalad2 | N-acetylated alpha-linked a... | NM_028279.3 | 36.4% | 36.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1017
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaatccagg ggccgtctgt acctttggat gtgcttggct gctgcgctgg 121 catctttcct gatgggattt atggtgggct ggtttattaa gcctctcaaa gaaacaacca 181 cttctgtgcg ctatcatcaa agtatacggt ggaaactggt atccgaaatg aaagctgaaa 241 acatcaaatc atttcttcgt tcttttacaa agcttcctca tctggcagga acagaacaaa 301 atttcttgct tgccaagaaa atccaaaccc agtggaagaa atttggacta gattcagcca 361 agttggttca ttatgatgtc ctcttatctt accccaatga gacaaatgcc aactatatat 421 cgattgtgga tgaacatgaa actgagattt tcaaaacatc ataccttgaa ccaccaccag 481 atggctatga gaatgttaca aatattgtgc caccatataa tgctttctca gcccaaggca 541 tgccagaggg agatcttgTA TATGTGAACT ATGCTCGCAC TGAAGACTTT TTCAAACTAG 601 AAAGAGAGAT GGGCATCAAC TGTACTGGGA AGATTGTTAT TGCAAGATAT GGAAAAATCT 661 TCAGAGGAAA TAAAGTTAAA AATGCCATGT TAGCAGGAGC CATAGGAATC ATCTTGTACT 721 CAGATCCAGC TGACTACTTT GCTCCTGAGG TACAGCCATA TCCCAAAGGA TGGAATCTTC 781 CTGGAACTGC AGCCCAGAGA GGAAATGTGT TAAATTTGAA TGGTGCTGGT GACCCACTCA 841 CTCCAGGCTA TCCAGCAAAA GAATACACTT TCAGACTTGA TGTTGAAGAA GGAGTGGGAA 901 TCCCCCGAAT ACCTGTACAT CCCATTGGAT ATAATGATGC AGAAATATTA TTACGGTATA 961 GTTTTCTTGT TGGATATGAG ATTAAGATAT TTTGCACTAG TTGTCTATTT TCTCATTGCC 1021 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1081 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1141 ATATATCTTG TGGAAAGGAC GATTCCGGTA TACTCTACCG GGTAACACGC GTTAAGTCga 1201 caatcaacct ctggattaca aaatttgtga aagatt