Construct: ORF TRCN0000469325
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008418.1_s317c1
- Derived from:
- ccsbBroadEn_04074
- DNA Barcode:
- AATCTTAATGATTTTACGCACATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYBC1 (79415)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469325
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NM_001033046.4 | 100% | 100% | |
| 2 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NM_001100407.3 | 100% | 100% | |
| 3 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NM_001193653.2 | 100% | 100% | |
| 4 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NM_001193654.2 | 100% | 100% | |
| 5 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NM_001193655.1 | 100% | 100% | |
| 6 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NM_001193657.2 | 100% | 100% | |
| 7 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | XM_017025074.2 | 100% | 100% | |
| 8 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | XM_017025076.1 | 100% | 100% | |
| 9 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | XM_017025077.1 | 100% | 100% | |
| 10 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NM_001100408.3 | 92.5% | 92.5% | 84_85ins42 |
| 11 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | XM_006722293.3 | 92.5% | 92.5% | 84_85ins42 |
| 12 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | XM_017025078.2 | 92.5% | 92.5% | 84_85ins42 |
| 13 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | XM_011523606.3 | 85% | 85% | 1_99del |
| 14 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | XM_017025073.2 | 78.6% | 78.6% | 1_99del;183_184ins42 |
| 15 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NR_036516.2 | 25.8% | (many diffs) | |
| 16 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NR_036517.2 | 23.8% | (many diffs) | |
| 17 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NR_036514.2 | 23.4% | 1_91del;214_215ins74;579_1999del | |
| 18 | human | 79415 | CYBC1 | cytochrome b-245 chaperone 1 | NR_036518.2 | 20.2% | (many diffs) | |
| 19 | mouse | 217370 | BC017643 | cDNA sequence BC017643 | NM_001252548.1 | 86.9% | 88.7% | (many diffs) |
| 20 | mouse | 217370 | BC017643 | cDNA sequence BC017643 | NM_001252549.1 | 86.9% | 88.7% | (many diffs) |
| 21 | mouse | 217370 | BC017643 | cDNA sequence BC017643 | NM_144832.2 | 86.9% | 88.7% | (many diffs) |
| 22 | mouse | 217370 | BC017643 | cDNA sequence BC017643 | NM_001254735.1 | 70.1% | 71.5% | (many diffs) |
| 23 | mouse | 217370 | BC017643 | cDNA sequence BC017643 | NM_001252550.1 | 57% | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 627
- ORF length:
- 561
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta cctgcaggtg gagacccgca ccagctcccg cctccatctg aagagggctc 121 caggcatccg gtcctggtcc ctgctggttg gaatcttgtc gattggcctg gctgctgcct 181 actacagcgg agatagcctg ggctggaagc tcttctacgt cacaggctgc ctgtttgtgg 241 ctgtgcagaa cttggaggac tgggaggaag ccaTCTTCGA CAAGAGCACA GGGAAGGTTG 301 TTTTGAAGAC GTTCAGCCTC TACAAGAAGC TGCTGACTCT TTTCAGAGCT GGCCACGACC 361 AGGTGGTGGT CCTGCTCCAT GATGTCCGTG ATGTGAGCGT GGAGGAGGAG AAGGTCCGGT 421 ACTTCGGGAA AGGCTACATG GTGGTGCTCC GGCTTGCGAC GGGCTTCTCC CACCCCCTCA 481 CGCAGAGTGC AGTCATGGGC CACCGCAGTG ATGTGGAAGC CATCGCCAAG CTCATCACCA 541 GCTTCCTGGA GCTGCACTGC CTTGAGAGCC CCACAGAGCT GTCTCAGAGC AGCGACAGTG 601 AGGCCGGTGA CCCTGCAAGC CAGAGCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 721 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAATCTTAA 781 TGATTTTACG CACATAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 841 aagatt