Construct: ORF TRCN0000469333
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015776.1_s317c1
- Derived from:
- ccsbBroadEn_06876
- DNA Barcode:
- AGCTTTGTATACAGGCAGATAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL8 (6132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469333
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6132 | RPL8 | ribosomal protein L8 | NM_000973.5 | 99.8% | 99.6% | 292A>G |
| 2 | human | 6132 | RPL8 | ribosomal protein L8 | NM_001317771.2 | 99.8% | 99.6% | 292A>G |
| 3 | human | 6132 | RPL8 | ribosomal protein L8 | NM_001317782.2 | 99.8% | 99.6% | 292A>G |
| 4 | human | 6132 | RPL8 | ribosomal protein L8 | NM_033301.3 | 99.8% | 99.6% | 292A>G |
| 5 | mouse | 26961 | Rpl8 | ribosomal protein L8 | NM_012053.2 | 87.5% | 99.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 837
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccgtgtgatc cgtggacaga ggaagggcgc cgggtctgtg ttccgcgcgc 121 acgtgaagca ccgtaaaggc gctgcgcgcc tgcgcgccgt ggatttcgct gagcggcacg 181 gctacatcaa gggcatcgtc aaggacatca tccacgaccc gggccgcggc gcgcccctcg 241 ccaaggtggt cttccgggat ccgtatcggt ttaagaagcg gacggagctg ttcattgccg 301 ccgagggcat tcacacgggc cagtttgtgt attgcggcaa gaaggcccag ctcaacgttG 361 GCAATGTGCT CCCTGTGGGC ACCATGCCTG AGGGTACAAT CGTGTGCTGC CTGGAGGAGA 421 AGCCTGGAGA CCGTGGCAAG CTGGCCCGGG CATCAGGGAA CTATGCCACC GTTATCTCCC 481 ACAACCCTGA GACCAAGAAG ACCCGTGTGA AGCTGCCCTC CGGCTCCAAG AAGGTTATCT 541 CCTCAGCCAA CAGAGCTGTG GTTGGTGTGG TGGCTGGAGG TGGCCGAATT GACAAACCCA 601 TCTTGAAGGC TGGCCGGGCG TACCACAAAT ATAAGGCAAA GAGGAACTGC TGGCCACGAG 661 TACGGGGTGT GGCCATGAAT CCTGTGGAGC ATCCTTTTGG AGGTGGCAAC CACCAGCACA 721 TCGGCAAGCC CTCCACCATC CGCAGAGATG CCCCTGCTGG CCGCAAAGTG GGTCTCATTG 781 CTGCCCGCCG GACTGGACGT CTCCGGGGAA CCAAGACTGT GCAGGAGAAA GAGAACTACC 841 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 901 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 961 ATATATCTTG TGGAAAGGAC GAAGCTTTGT ATACAGGCAG ATAGGTACGC GTTAAGTCga 1021 caatcaacct ctggattaca aaatttgtga aagatt