Construct: ORF TRCN0000469340
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018960.3_s317c1
- Derived from:
- ccsbBroadEn_15253
- DNA Barcode:
- CCCGGACATTGGAGAATAGCCTAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MYO3B (140469)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469340
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140469 | MYO3B | myosin IIIB | NM_138995.5 | 99.8% | 81.2% | (many diffs) |
| 2 | human | 140469 | MYO3B | myosin IIIB | XM_006712299.4 | 99.1% | 80.6% | (many diffs) |
| 3 | human | 140469 | MYO3B | myosin IIIB | NM_001083615.4 | 97.8% | 83.4% | (many diffs) |
| 4 | human | 140469 | MYO3B | myosin IIIB | XM_011510655.3 | 97.1% | 82.8% | (many diffs) |
| 5 | human | 140469 | MYO3B | myosin IIIB | XM_011510654.3 | 96.5% | 78.5% | (many diffs) |
| 6 | human | 140469 | MYO3B | myosin IIIB | XM_011510656.3 | 74.8% | 57.4% | (many diffs) |
| 7 | human | 140469 | MYO3B | myosin IIIB | XM_011510657.3 | 73.5% | 55.4% | (many diffs) |
| 8 | human | 140469 | MYO3B | myosin IIIB | XM_011510658.3 | 73.5% | 55.4% | (many diffs) |
| 9 | human | 140469 | MYO3B | myosin IIIB | XM_024452713.1 | 73.5% | 55.4% | (many diffs) |
| 10 | human | 140469 | MYO3B | myosin IIIB | NR_045683.2 | 64.3% | (many diffs) | |
| 11 | human | 140469 | MYO3B | myosin IIIB | NR_045682.2 | 63.4% | (many diffs) | |
| 12 | human | 140469 | MYO3B | myosin IIIB | NR_045684.2 | 63% | (many diffs) | |
| 13 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319039.1 | 81.8% | 75.4% | (many diffs) |
| 14 | mouse | 329421 | Myo3b | myosin IIIB | NM_177376.4 | 81.6% | 75.3% | (many diffs) |
| 15 | mouse | 329421 | Myo3b | myosin IIIB | XM_006499745.3 | 80.8% | 74.4% | (many diffs) |
| 16 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319037.1 | 80.8% | 74.4% | (many diffs) |
| 17 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319038.1 | 80% | 73.7% | (many diffs) |
| 18 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319040.1 | 79.5% | 72.9% | (many diffs) |
| 19 | mouse | 329421 | Myo3b | myosin IIIB | XM_006499750.3 | 78.7% | 71.9% | (many diffs) |
| 20 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319041.1 | 75.9% | 79% | (many diffs) |
| 21 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319042.1 | 75.2% | 79.3% | (many diffs) |
| 22 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319043.1 | 75.1% | 79.6% | (many diffs) |
| 23 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319044.1 | 74.1% | 82.7% | (many diffs) |
| 24 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319045.1 | 56% | 72.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3399
- ORF length:
- 3330
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaacatctg tatggattat ttcactataa tcctatgatg cttggacttg 121 aatcacttcc agatcccaca gacacctggg aaattataga gaccattggt aaaggcacct 181 atggcaaagt ctacaaggta actaacaaga gagatgggag cctggctgca gtgaaaattc 241 tggatccagt cagtgatatg gatgaagaaa ttgaggcaga atacaacatt ttgcagttcc 301 ttcctaatca tcccaatgtt gtaaagtttt atgggatgtt ttacaaagcg gatcactgtg 361 tagggggaca gctgtggctg gtcctggagc tgtgtaatgg gggctcagtc actgagcttg 421 tcaaaggtct actcagatgt ggccagcggt tggatgaagc aatgatctca tacatcttgt 481 acggggccct cttgggcctt cagcatttgc acaacaaccg aatcatccac cgtgatgtga 541 aggggaataa cattcttctg acaacagaag gaggagttaa gctcgttgac tttggtgttt 601 cagctcaact caccagtaca cgtctgcgga gaaacacatc tgttggcacc ccgttctgga 661 tggcccctga ggtcattgcc tgtgagcagc agtatgactc ttcctatgac gctcgctgtg 721 acgtctggtc cttggggatc acagctattg aactggggga tggagaccct cccctctttg 781 acatgcatcc tgtgaaaaca ctctttaaga ttccaagaaa tcctccacct actttacttc 841 atccagaaaa atggtgtgaa gaattcaacc actttatttc acagtgtctt attaaggatt 901 ttgaaaggcg accttccgtc acacatctcc ttgaccaccc atttattaaa ggagtacatg 961 gaaaagttct gtttctgcaa aaacagctgg ccaaggttct ccaagaccag aagcatcaaa 1021 atcctgttgc taaaaccagg catgagagga tgcataccag aagaccttat catgtggaag 1081 atgctgaaaa atactgcctt gaggatgatt tggtcaacct agaggttctg gatgaggata 1141 caattatcca tcagttgcag aaacgttatg cagacttgct aatttacaca tatgttggag 1201 acatcttaat tgccttaaac cccttccaga atctaagcat atactctcca cagttttcca 1261 gactttatca tggggtgaaa cgcgcctcca atccccccca catatttgca tcagcagatg 1321 ctgcttacca gtgcatggtt actctcagca aagaccagtg cattgtcatc agcggagaga 1381 gtggctctgg gaagacagaa agcgcccacc tgattgttca acatttgact ttcttgggaa 1441 aggccaataa tcagaccttg agagagaaaa ttctacaagt caactccctg gtggaagcct 1501 ttgggaactc atgcactgcc atcaatgaca actcgagccg ttttggaaaa tatctggaaa 1561 tgatgtttac accaactgga gttgtgatgg gggcaagaat ctctgaatat ctcctggaaa 1621 aatccagagt tataaaacag gcagcgagag agaaaaattt tcatatattt tactatattt 1681 atgctggtct tcatcaccaa aagaagcttt ctgatttcag acttcctgag gaaaaacctc 1741 ctaggtacat agctgatgaa actggaaggg tgatgcacga cataacttcc aaggagtctt 1801 acagaagaca attcgaagca attcagcatt gcttcaggat tatagggttc acggacaaag 1861 aggtgcactc agtgtacaga attttggctg ggattttgaa tattgggaac attgagttcg 1921 cagctatttc ctctcaacat cagactgata aaagtgaggt gcccaatgct gaagctttgc 1981 aaaatgctgc ctctgttctg tgcattagcc ctgaagagct ccaggaggcc ctcacctccc 2041 actgtgtggt cacccggggc gagaccatca tccgtgccaa cactgtagac agggctgcgg 2101 acgttcgaga cgccatgtcc aaagccctgt atgggaggct cttcagctgg attgtgaatc 2161 gcattaatac actcctgcag ccagacgaaa acatatgtag tgcaggaggt ggaatgaatg 2221 tggggatctt ggatatcttt ggattcgaga attttcagag aaattcattt gagcagctct 2281 gcataaacat cgccaatgag caaatccagt actatttcaa tcagcatgtt tttgctcttg 2341 agcagatgga atatcagaat gaaggcattg atgctgtacc cgtggaatat gaggacaacc 2401 gcccgctctt ggacatgttc ctccagaaac ccctgggact gcttgcactt ttggatgagg 2461 aaagtcggtt tccccaagca actgaccaga ccctggttga taaatttgaa gataatctac 2521 gatgcaaata cttctggagg cccaaaggag tggaactgtg ctttggcatt cagcattatg 2581 ctggaaaggt attatatgat gcttctgggg ttcttgagaa aaatagagac actctccctg 2641 ccgatgtggt tgtggtcctg agaacgtcag aaaacatgct tcttcagcag ctcttctcaa 2701 tccctctgac caaaacaggt aatttggccc agacaagagc taggataaca gtggcctcaa 2761 gttctttgcc tccacatttc agtgctggga aagccaaggt ggacactctg gaggtgatac 2821 ggcatccgga agaaaccacc aacatgaaga ggcaaactgt ggcttcttac ttccggtatt 2881 ctctgatgga cctgctctcc aaaatggtgg ttggacagcc ccactttgtg cgctgcatta 2941 aacccaatga tgaccgagag gccctgcagt tctctcgaga gagggtgctg gcccagctcc 3001 gctccacagg gattctggag acagtcagca tccgccgcca gggctattcc caccgcatcc 3061 tttttgaaga atttgtgaaa aggtattatt acttggcatt cacagcacat caaacacctc 3121 ttgctagcaa agagagctgt gtggctatct tggaaaagtc cagattagat cactgggtgc 3181 tgggaaaaac aaaggttttt ctcaaatatt accatgttga gcaattaaat ttgctgcttc 3241 gagaagtcat aggcagagtg gttgtgctgc aggcatatac caaggggtgg cttggagcca 3301 ggagatacaa aaaggtcaga gagaagagag agaagggagc cattgccatc ccagtcagcc 3361 ttggagagga tatgatgctc ggaggaaatt taagaaaata agcaacagaa ggaatgagtc 3421 tgctgctcat aatcaagcag gggacacttc aaaccaaagc agtgggccac attcccccgt 3481 cgcagcaggt acgaggggaa gtgccgaggt tcaagactgc agcgagcctg gtgaccataa 3541 agttctcagg ggctctgtac atcgtaggag ccattcacaa gcagaatcca acaatggccg 3601 tacacagact tcaagcaact ctcctgctgt cacagagaaa aatgggcatt cacaagccca 3661 gagttctcca aaagggtgcg atatcttcgc aggacatgca aacaagcact cggtttctgg 3721 gactgatttg ctgtcttctc ggatatgcca tccTGCTCCA GATCAGCAAG GATTGAGTCT 3781 CTGGGGAGCC CCTCAAAAGC CTGGTTCAGA AAATGGTCTT GCACAGAAGC ATCGAACACC 3841 TCGCCGACGA TGTCAGCAGC CCAAAATGCT GAGTAGCCCT GAGGACACCA TGTACTATAA 3901 CCAGTTAAAT GGAACTCTAG AATATCAAGG GAGCAAGAGG AAGCCAAGAA AACTTGGCCA 3961 AATCAAAGTA CTTGATGGGG AAGATGAATA TTACAAATCT CTGTCACCAG TGGACTGTAT 4021 CCCTGAGGAG AACAACTCAG CCCACCCTTC CTTTTTTTCT TCATCCTCAA AAGGAGACTC 4081 TTTTGCTCAA CATTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 4141 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 4201 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CCGGACATTG GAGAATAGCC 4261 TAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att