Construct: ORF TRCN0000469357
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009011.1_s317c1
- Derived from:
- ccsbBroadEn_06038
- DNA Barcode:
- TGTCATACTTCCAAGAGTACCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRK (1398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469357
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1398 | CRK | CRK proto-oncogene, adaptor... | NM_016823.4 | 99.8% | 100% | 49A>C |
| 2 | human | 1398 | CRK | CRK proto-oncogene, adaptor... | NM_005206.5 | 66.8% | 66.7% | (many diffs) |
| 3 | mouse | 12928 | Crk | v-crk avian sarcoma virus C... | NM_133656.5 | 96.2% | 99.6% | (many diffs) |
| 4 | mouse | 12928 | Crk | v-crk avian sarcoma virus C... | XM_006532124.2 | 82.5% | 85.5% | (many diffs) |
| 5 | mouse | 12928 | Crk | v-crk avian sarcoma virus C... | XM_006532125.2 | 72.5% | 65% | (many diffs) |
| 6 | mouse | 12928 | Crk | v-crk avian sarcoma virus C... | NM_001277219.1 | 63.4% | 66.4% | (many diffs) |
| 7 | mouse | 12928 | Crk | v-crk avian sarcoma virus C... | NM_001277221.1 | 25.3% | 26.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 978
- ORF length:
- 912
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggcaacttc gactcggagg agcggagtag ctggtactgg gggcggttga 121 gtcggcagga ggcggtggcg ctgctgcagg gccagcggca cggggtgttc ctggtgcggg 181 actcgagcac cagccccggg gactatgtgc tcagcgtctc agagaactcg cgcgtctccc 241 actacatcat caacagcagc ggcccgcgcc cgccggtgcc accgtcgccc gcccagcctc 301 cgcccggggt gagcccctcc agactccgaa taggagatca agagtttgat tcattgcctg 361 ctttactgga attctacaaa atacactatt tggacactac aacgttgata gaaccagttt 421 ccagatccag gcagggtagt ggagtgattc tcaggcagga ggaggcggag tatgtgcgag 481 ccctctttga ctttaatggg aatgatgagg aagatcttcc ctttaagaaa ggagacatct 541 tgagaatccg ggacaagccT GAAGAGCAGT GGTGGAATGC GGAGGACAGC GAAGGCAAGA 601 GAGGGATGAT TCCAGTCCCT TACGTCGAGA AGTATAGACC TGCCTCCGCC TCAGTATCGG 661 CTCTGATTGG AGGTAACCAG GAGGGTTCCC ACCCACAGCC ACTGGGTGGG CCGGAGCCTG 721 GGCCCTATGC CCAACCCAGC GTCAACACTC CGCTCCCTAA CCTCCAGAAT GGGCCCATAT 781 ATGCCAGGGT TATCCAGAAG CGAGTCCCCA ATGCCTACGA CAAGACAGCC TTGGCTTTGG 841 AGGTCGGTGA GCTGGTAAAG GTTACGAAGA TTAATGTGAG TGGTCAGTGG GAAGGGGAGT 901 GTAATGGCAA ACGAGGTCAC TTCCCATTCA CACATGTCCG TCTGCTGGAT CAACAGAATC 961 CCGATGAGGA CTTCAGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1021 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1081 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGTCATA CTTCCAAGAG 1141 TACCAACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt