Construct: ORF TRCN0000469360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014414.1_s317c1
- Derived from:
- ccsbBroadEn_08964
- DNA Barcode:
- GACTACAACCTCGTCTCCTTTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOB3B (79817)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469360
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79817 | MOB3B | MOB kinase activator 3B | NM_024761.5 | 99.8% | 99.5% | 455A>T |
| 2 | mouse | 214944 | Mob3b | MOB kinase activator 3B | NM_178061.5 | 90.4% | 97.6% | (many diffs) |
| 3 | mouse | 214944 | Mob3b | MOB kinase activator 3B | XM_006537752.3 | 90.4% | 97.6% | (many diffs) |
| 4 | mouse | 214944 | Mob3b | MOB kinase activator 3B | XM_006537753.2 | 90.4% | 97.6% | (many diffs) |
| 5 | mouse | 214944 | Mob3b | MOB kinase activator 3B | XM_006537754.2 | 90.4% | 97.6% | (many diffs) |
| 6 | mouse | 214944 | Mob3b | MOB kinase activator 3B | XM_006537755.3 | 88.2% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 714
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc catagccctg aagcaggtat tcaacaagga caagaccttc cgacccaaga 121 ggaaatttga acctggcaca cagaggtttg agctgcacaa acgggctcag gcatccctca 181 actcgggtgt ggacctgaag gcggctgtgc agttgcccag tggggaggac cagaatgact 241 gggtggcagt acatgtggtg gacttcttca atcggatcaa cctcatctat ggcaccatct 301 gtgagttcTG CACCGAGCGG ACCTGTCCTG TGATGTCAGG GGGCCCCAAA TATGAGTATC 361 GGTGGCAGGA TGATCTCAAG TATAAGAAGC CAACAGCGCT GCCAGCTCCC CAGTACATGA 421 ACCTTCTTAT GGATTGGATT GAGGTTCAGA TCAACAACGA GGAAATATTT CCAACATGCG 481 TGGGTGTTCC CTTCCCAAAG AACTTCCTTC AGATCTGCAT GAAGATCCTG TGCCGCCTTT 541 TCCGGGTCTT TGTCCACGTC TATATCCACC ACTTCGACCG GGTCATTGTG ATGGGTGCAG 601 AGGCCCATGT CAACACCTGC TACAAACACT TCTATTACTT TGTCACAGAG ATGAACCTCA 661 TAGACCGCAA GGAGCTAGAG CCTTTGAAAG AAATGACGAG CAGGATGTGT CACTACCCAA 721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAG ACTACAACCT CGTCTCCTTT AACACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att