Transcript: Mouse XM_006537754.2

PREDICTED: Mus musculus MOB kinase activator 3B (Mob3b), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mob3b (214944)
Length:
2238
CDS:
268..918

Additional Resources:

NCBI RefSeq record:
XM_006537754.2
NBCI Gene record:
Mob3b (214944)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006537754.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000088785 CATCACTTTGACCGTGTCATT pLKO.1 769 CDS 100% 4.950 6.930 N Mob3b n/a
2 TRCN0000379391 CAAACGTGGAGCTACCATATA pLKO_005 1338 3UTR 100% 13.200 10.560 N Mob3b n/a
3 TRCN0000088786 TCATCACTTTGACCGTGTCAT pLKO.1 768 CDS 100% 4.950 3.960 N Mob3b n/a
4 TRCN0000363370 TAACTTCTCTGTCACTTATTT pLKO_005 1302 3UTR 100% 15.000 10.500 N Mob3b n/a
5 TRCN0000364268 GCAGGATGACCTCAAGTATAA pLKO_005 567 CDS 100% 13.200 9.240 N Mob3b n/a
6 TRCN0000088784 CCAGTACATGAATCTGCTTAT pLKO.1 612 CDS 100% 10.800 7.560 N Mob3b n/a
7 TRCN0000419466 ACAGAGATGAACCTCATAGAC pLKO_005 847 CDS 100% 4.950 3.465 N MOB3B n/a
8 TRCN0000379370 TGGACTTCTTCAATCGCATTA pLKO_005 461 CDS 100% 10.800 6.480 N Mob3b n/a
9 TRCN0000434590 ACAAACACTTCTATTACTTTG pLKO_005 824 CDS 100% 10.800 7.560 N MOB3B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006537754.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08964 pDONR223 100% 90.4% 97.6% None (many diffs) n/a
2 ccsbBroad304_08964 pLX_304 0% 90.4% 97.6% V5 (many diffs) n/a
3 TRCN0000469360 GACTACAACCTCGTCTCCTTTAAC pLX_317 62.2% 90.4% 97.6% V5 (many diffs) n/a
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