Construct: ORF TRCN0000469365
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018209.1_s317c1
- Derived from:
- ccsbBroadEn_03632
- DNA Barcode:
- CACACTCTTAGACTCCCATACTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VAC14 (55697)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469365
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | NM_018052.5 | 100% | 100% | |
| 2 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_005256038.4 | 94.3% | 91.6% | (many diffs) |
| 3 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_011523224.3 | 79.7% | 70.1% | (many diffs) |
| 4 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_011523223.3 | 79.3% | 70.8% | (many diffs) |
| 5 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_005256041.4 | 79.2% | 72.5% | (many diffs) |
| 6 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_017023441.2 | 79.1% | 68.1% | (many diffs) |
| 7 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_011523225.3 | 74.5% | 70.9% | (many diffs) |
| 8 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | XM_017023442.2 | 73.6% | 71.5% | (many diffs) |
| 9 | human | 55697 | VAC14 | VAC14 component of PIKFYVE ... | NM_001351157.2 | 70% | 70% | 0_1ins702 |
| 10 | mouse | 234729 | Vac14 | Vac14 homolog (S. cerevisiae) | NM_146216.2 | 89.3% | 93.9% | (many diffs) |
| 11 | mouse | 234729 | Vac14 | Vac14 homolog (S. cerevisiae) | XM_006530918.2 | 77.4% | 81.4% | (many diffs) |
| 12 | mouse | 234729 | Vac14 | Vac14 homolog (S. cerevisiae) | XM_006530919.3 | 63.7% | 66.1% | (many diffs) |
| 13 | mouse | 234729 | Vac14 | Vac14 homolog (S. cerevisiae) | XR_001778432.1 | 63.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2415
- ORF length:
- 2346
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaccccgag aaggatttcg cgccgctcac gcctaacatc gtgcgcgccc 121 tcaatgacaa gctgtacgaa aagcggaagg tggcagcgct ggagatcgag aagctggtcc 181 gggagttcgt ggcccagaac aataccgtgc aaatcaagca tgtgatccag accctgtccc 241 aggagtttgc cctgtctcag cacccccaca gccggaaagg gggcctcatc ggcctggccg 301 cctgctccat cgcactgggc aaggactcag ggctctacct gaaggagctg atcgagccag 361 tgctgacctg cttcaatgat gcagacagca ggctgcgcta ctatgcctgc gaggccctct 421 acaacatcgt caaggtggcc cggggcgctg tgctgcccca cttcaacgtg ctctttgacg 481 ggctgagcaa gctggcagcc gacccagacc ccaatgtgaa aagcggatct gagctcctag 541 accgcctttt aaaggacatt gtgactgaga gcaacaagtt tgacctggtg agcttcatcc 601 ccttgttgcg agagaggatt tactccaaca accagtatgc ccggcagttc atcatctcct 661 ggatcctggt tctggagtcg gtgccagaca ttaacctgct ggattacctg ccggagatcc 721 tggatggact cttccagatc ctgggtgaca atggcaaaga gattcgcaaa atgtgtgagg 781 ttgttcttgg agaattctta aaagaaatta agaagaaccc ctccagtgtg aagtttgctg 841 agatggccaa catcctggtg atccactgcc agacaacaga tgacctcatc cagctgacag 901 ccatgtgctg gatgcgggag ttcatccagc tggcgggccg cgtcatgctg ccttactcct 961 ccgggatcct gactgctgtc ttgccctgct tggcctacga tgaccgcaag aaaagcatca 1021 aagaagtggc caacgtgtgc aaccagagcc tgatgaagct ggtcaccccc gaggacgacg 1081 agctggatga gctgagacct gggcagaggc aggcagagcc cacccctgac gatgccctgc 1141 caaagcagga gggcacagcc agtggaggtc cagatggttc ctgtgactcc agcttcagta 1201 gcggcatcag tgtcttcact gcagccagca ctgaaagagc cccagtgacc cttcacctcg 1261 acgggatcgt gcaggtccta aactgccacc tcagtgacac ggccattggg atgatgacca 1321 ggattgcagt tctcaagtgg ctctaccacc tctacatcaa aactcctcgg aagatgttcc 1381 ggcacacgga cagcctcttt cccatcctac tgcagacgtt atcggatgaa tcggatgagg 1441 tgatcctgaa ggacctggag gtgctggcag aaatcgcttc ctcccccgca ggccagacgg 1501 atgacccagg ccccctcgat ggccctgacc tccaggccag ccactcagag ctccaggtgc 1561 ccacccctgg cagagccggc ctactgaaca cctctggtac caaaggctta gaatgttctc 1621 cttcaactcc caccatgaat tcttactttt ataagttcat gatcaacctt ctcaagagat 1681 tcagcagcga acggaagctc ctggaggtca gaggcccttt catcatcagg cagctgtgcc 1741 tcctgctgaa tgcggagaac atcttccact caatggcaga catcctgctg cgggaggagg 1801 acctcaagtt cgcctcgacc atggtccacg ccctcaacac catcctgctg acctccacag 1861 agctcttcca gctaaggaac cagctgaagg acctgaagac cctggagagc cagaacctgt 1921 tctgctgcct gtaccgctcc tggtgccaca acccagtcac cacggtgtcc ctctgcttcc 1981 tcacccagaa ctaccggcac gcctatgacc tcatccagaa gtttggggac cTGGAGGTCA 2041 CCGTGGACTT CCTCGCAGAG GTGGACAAGC TGGTGCAGCT GATTGAGTGC CCCATCTTCA 2101 CATATCTGCG CCTGCAGCTG CTGGACGTGA AGAACAACCC CTACCTGATC AAGGCCCTCT 2161 ACGGCCTGCT CATGCTCCTG CCGCAGAGCA GCGCCTTCCA GCTGCTCTCG CACCGGCTCC 2221 AGTGCGTGCC CAACCCTGAG CTGCTGCAGA CCGAAGACAG TCTAAAGGCA GCCCCCAAGT 2281 CCCAGAAAGC TGACTCCCCT AGCATCGACT ACGCAGAGCT GCTGCAGCAC TTTGAGAAGG 2341 TCCAGAACAA GCACCTGGAA GTGCGGCACC AGCGGAGCGG GCGTGGGGAC CACCTGGACC 2401 GGAGGGTTGT CCTCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2461 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2521 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CACACTCTTA GACTCCCATA 2581 CTGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt