Construct: ORF TRCN0000469386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014696.1_s317c1
- Derived from:
- ccsbBroadEn_06828
- DNA Barcode:
- GACCCGACGTAATGTCCATTCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PYCR1 (5831)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469386
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_001282280.1 | 99.8% | 100% | 576T>C |
| 2 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_006907.4 | 99.7% | 100% | 576T>C;903A>G |
| 3 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | XM_005256381.2 | 99.7% | 100% | 576T>C;903A>G |
| 4 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | XM_011523583.2 | 99.7% | 100% | 576T>C;903A>G |
| 5 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | XM_011523584.3 | 99.7% | 100% | 576T>C;903A>G |
| 6 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | XM_024450849.1 | 99.7% | 100% | 576T>C;903A>G |
| 7 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_153824.3 | 93.2% | 93.7% | (many diffs) |
| 8 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_001282281.2 | 92% | 92.1% | 1_81del;657T>C;984A>G |
| 9 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_001282279.2 | 90.1% | 90.2% | 540_541ins93;810A>G |
| 10 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | NM_001330523.1 | 67.2% | 67.3% | (many diffs) |
| 11 | human | 5831 | PYCR1 | pyrroline-5-carboxylate red... | XM_011523585.2 | 62% | 62.1% | (many diffs) |
| 12 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | NM_001348222.1 | 83.7% | 88.7% | (many diffs) |
| 13 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | NM_144795.3 | 83.7% | 88.7% | (many diffs) |
| 14 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | XM_006532735.2 | 83.7% | 88.7% | (many diffs) |
| 15 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | XM_011248855.2 | 83.7% | 88.7% | (many diffs) |
| 16 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | XM_011248854.2 | 76.5% | 81% | (many diffs) |
| 17 | mouse | 209027 | Pycr1 | pyrroline-5-carboxylate red... | XM_011248856.2 | 75.7% | 79.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1023
- ORF length:
- 957
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgtgggcttc atcggcgctg gccagctggc ttttgccctg gccaagggct 121 tcacagcagc aggcgtcttg gctgcccaca agataatggc tagctcccca gacatggacc 181 tggccacagt ttctgctctc aggaagatgg gggtgaagtt gacaccccac aacaaggaga 241 cggtgcagca cagtgatgtg ctcttcctgg ctgtgaagcc acacatcatc cccttcatcc 301 tggatgaaat aggcgccgac attgaggaca gacacattgt ggtgtcctgc gcggccggcg 361 tcaccatcag ctccattgag aagaagctgt cagcgtttcg gccagccccc agggtcatcc 421 gctgcatgac caacactcca gtcgtggtgc gggagggggc caccgtgtat gccacaggca 481 cgcacgccca ggtggaggac gggaggctca tggagcagct gctgagcagc gtgggcttct 541 gcacggaggt ggaagaggac ctgattgatg ccgtcacggg gctcagtggc agcggccccg 601 cctacgcatt cacagccctg gatgccctgg ctgatggggg cgtgaagatg ggacttccaa 661 ggcgcctggc agtccgcctc ggggcccagg cccTCCTGGG GGCTGCCAAG ATGCTGCTGC 721 ACTCAGAACA GCACCCAGGC CAGCTCAAGG ACAACGTCAG CTCTCCTGGT GGGGCCACCA 781 TCCATGCCTT GCATGTGCTG GAGAGTGGGG GCTTCCGCTC CCTGCTCATC AACGCTGTGG 841 AGGCCTCCTG CATCCGCACA CGGGAGCTGC AGTCCATGGC TGACCAGGAG CAGGTGTCAC 901 CAGCCGCCAT CAAGAAGACC ATCCTGGACA AGGTGAAGCT GGACTCCCCT GCAGGGACCG 961 CTCTGTCGCC TTCTGGCCAC ACCAAGCTGC TCCCCCGCAG CCTGGCCCCA GCGGGCAAGG 1021 ATTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1081 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1141 GGCTTTATAT ATCTTGTGGA AAGGACGAGA CCCGACGTAA TGTCCATTCT TGACGCGTTA 1201 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt