Construct: ORF TRCN0000469395

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005731.1_s317c1
Derived from:
ccsbBroadEn_11586
DNA Barcode:
ATTAGGCCTGGAATAATTAAGGAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CPSF6 (11052)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469395
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11052 CPSF6 cleavage and polyadenylatio... NM_001300947.2 100% 100%
2 human 11052 CPSF6 cleavage and polyadenylatio... XM_005268588.3 99.8% 99.8% 1578_1580delAAG
3 human 11052 CPSF6 cleavage and polyadenylatio... NM_007007.3 93.7% 93.7% 692_693ins111
4 human 11052 CPSF6 cleavage and polyadenylatio... XM_005268590.3 93.5% 93.5% 692_693ins111;1467_1469delAAG
5 mouse 432508 Cpsf6 cleavage and polyadenylatio... XM_006513854.3 93% 99.6% (many diffs)
6 mouse 432508 Cpsf6 cleavage and polyadenylatio... XM_006513855.2 93% 99.6% (many diffs)
7 mouse 432508 Cpsf6 cleavage and polyadenylatio... XM_006513851.3 92.9% 99.4% (many diffs)
8 mouse 432508 Cpsf6 cleavage and polyadenylatio... XM_006513852.2 92.9% 99.4% (many diffs)
9 mouse 432508 Cpsf6 cleavage and polyadenylatio... XM_006513853.2 92.9% 99.4% (many diffs)
10 mouse 432508 Cpsf6 cleavage and polyadenylatio... NM_001013391.2 86.7% 93.3% (many diffs)
11 mouse 432508 Cpsf6 cleavage and polyadenylatio... XM_006513858.3 86.7% 93.3% (many diffs)
12 mouse 432508 Cpsf6 cleavage and polyadenylatio... NM_001310609.1 86.6% 93.2% (many diffs)
13 mouse 432508 Cpsf6 cleavage and polyadenylatio... XM_006513856.3 86.6% 93.2% (many diffs)
14 mouse 432508 Cpsf6 cleavage and polyadenylatio... XR_001779551.1 12.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1830
ORF length:
1764
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggacggcgtg gaccacatag acatttacgc ggatgtcggc gaagagttca
121accaggaagc tgaatatggt gggcatgatc agatagattt gtatgacgat gtcatatctc
181catctgcaaa taatggagat gccccagaag accgagatta catggatact ctcccaccaa
241ctgttggtga tgatgtgggt aaaggagcag caccaaatgt tgtctataca tatactggaa
301agagaattgc attatatatt ggaaatctaa catggtggac aacagatgaa gacttaactg
361aagcagttca ttctttggga gtaaatgata ttttggagat aaaatttttt gaaaatcggg
421caaatggcca gtcaaagggg tttgcccttg ttggtgttgg atctgaagca tcttcaaaaa
481agttaatgga tctgttacct aaaagagaac ttcatggtca gaatcctgtt gtaactccat
541gcaataaaca gttcctgagt caatttgaaa tgcagtccag gaaaactaca caatcaggac
601aaatgtctgg ggaaggtaaa gctggtcctc caggaggcag ttcccgtgca gcatttccac
661aaggtggtag aggacggggc cgttttccag gggctgttcc tggtggggac agatttcctg
721ggccagcagg accaggaggg ccacccccac cttttccagg aaatttgatc aagcatcttg
781ttaaaggaac tcggcctttg ttcctggaaa ctaggattcc atggcatatg gggcacagca
841tagaggaaat acccattttt ggcctaaaag ctggacagac tccaccacgt ccacccttag
901gtcctccagg cccacctggt ccaccaggtc ctccacctcc tggtcaggtt ctgcctcctc
961ctctagctgg gcctcctaat cgaggagatc gccctccacc accagttctt tttcctggac
1021aaccttttgg gcagcctcca ttgggtccac ttcctcctgg ccctccacct ccagttccag
1081gctacggccc ccctcctggc ccaccacctc cacaacaggg accacctcca cctccaggcc
1141cctttccacc tcgtccaccc ggtccacttg ggccacccct tacactagct cctcctccgc
1201atcttcctgg accacctcca ggtgccccac cgccagctcc gcatgtgaac ccagctttct
1261ttcctccacc aactaacagt ggcatgccta catcagatag ccgaggtcca ccaccaacag
1321atccatatgg gcgacctcca ccatatgata ggggtgacta tggcccccct ggaagggaaa
1381tggatactgc aagaacgcca ttgagtgaag ctgaatttga agaaatcatg aatagaaata
1441gggcaaTCTC AAGCAGTGCT ATTTCGAGAG CTGTGTCTGA TGCCAGTGCT GGTGATTATG
1501GGAGTGCTAT TGAGACACTG GTAACTGCAA TTTCTTTAAT TAAACAATCC AAAGTATCTG
1561CTGATGATCG TTGCAAAGTT CTTATTAGTT CTTTGCAAGA TTGCCTTCAT GGAATTGAGT
1621CCAAGTCTTA TGGTTCTGGA TCAAGACGTG AACGATCAAG AGAGAGGGAC CATAGTAGAT
1681CACGAGAAAA GAGTCGACGT CATAAATCCC GTAGTAGAGA CCGTCATGAC GATTATTACA
1741GAGAGAGAAG CAGAGAACGA GAGAGGCACC GGGATCGTGA CCGAGACCGT GACCGAGAGC
1801GTGACCGAGA GCGCGAATAT CGTCATCGTT ACCCAACTTT CTTGTACAAA GTGGTTGATA
1861TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1921CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATTAG
1981GCCTGGAATA ATTAAGGAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
2041tgaaagatt

Download FASTA (ORF) (Full)