Construct: ORF TRCN0000469408
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005009.1_s317c1
- Derived from:
- ccsbBroadEn_01544
- DNA Barcode:
- GTATGTTGGACATGTTCAGGTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC2A5 (6518)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469408
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6518 | SLC2A5 | solute carrier family 2 mem... | NM_001135585.2 | 100% | 100% | |
| 2 | human | 6518 | SLC2A5 | solute carrier family 2 mem... | NM_001328619.2 | 45.4% | 38% | (many diffs) |
| 3 | human | 6518 | SLC2A5 | solute carrier family 2 mem... | NM_003039.3 | 45.4% | 38% | (many diffs) |
| 4 | human | 6518 | SLC2A5 | solute carrier family 2 mem... | NM_001328620.2 | 36.7% | 27.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcaacaggat cagagcatga aggaagggag gctgacgctt gtgcttgccc 121 tggcaaccct gatagctgcc tttgggtcat ccttccagta tgggtacaac gtggctgctg 181 tcaactcccc agcactgctc atgcaacaat tttacaatga gacttactat ggtaggaccg 241 gtgaattcat ggaagacttc cccttgacgt tgctgtggtc tgtaaccgtg tccatgtttc 301 catttggagg gtttatcgga tccctcctgg tcggcccctt ggtgaataaa tttggcagaa 361 aaggggcctt gctgttcaac aacatatttt ctatcgtgcc tgcgatctta atgggatgca 421 gcagagtcgc cacatcattt GAGCTTATCA TTATTTCCAG ACTTTTGGTG GGAATATGTG 481 CAGGTGTATC TTCCAACGTG GTCCCCATGT ACTTAGGGGA GCTGGCCCCT AAAAACCTGC 541 GGGGGGCTCT CGGGGTGGTG CCCCAGCTCT TCATCACTGT TGGCATCCTT GTGGCCCAGA 601 TCTTTGGTCT TCGGAATCTC CTTGCAAACG TAGATGGTGA GTTCAGGACA TCTCGGGAGC 661 ACCCCCACCC CTTCACCACT ACCCTTGGCC CCCTCCTTGT GTTCCAAAGC CACCACCACA 721 GGACAGGACT TTCTGCAGAC TGGTCTCTTC TAACAGGCTG GATGTCCTTG GGGGGCCCAT 781 CCTGTCCCGA GCCAACATAC TCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 841 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 901 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTATGTT GGACATGTTC 961 AGGTGTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt