Construct: ORF TRCN0000469422
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009893.1_s317c1
- Derived from:
- ccsbBroadEn_07970
- DNA Barcode:
- TGTCGGGTTGATTCAGGCCTCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RWDD3 (25950)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469422
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25950 | RWDD3 | RWD domain containing 3 | NM_001128142.1 | 99.6% | 98.9% | 226A>G;258T>G |
| 2 | human | 25950 | RWDD3 | RWD domain containing 3 | NM_001199682.1 | 90.3% | 86.8% | (many diffs) |
| 3 | human | 25950 | RWDD3 | RWD domain containing 3 | NM_001278247.1 | 81.2% | 71.6% | (many diffs) |
| 4 | human | 25950 | RWDD3 | RWD domain containing 3 | NM_015485.5 | 72.5% | 70.7% | (many diffs) |
| 5 | human | 25950 | RWDD3 | RWD domain containing 3 | NM_001278248.1 | 65.6% | 59.8% | (many diffs) |
| 6 | human | 25950 | RWDD3 | RWD domain containing 3 | NR_103483.1 | 46% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 654
- ORF length:
- 585
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggagcct gtgcaggagg agctctcggt cctggccgcg attttctgca 121 ggccccacga gtgggaggtg ctgagccgct cagagacaga tgggaccgtg ttcagaattc 181 acacaaaagc tgaaggattt atggatgcgg atatacctct ggaattggtg ttccatttgc 241 cagtcaatta tccttcatgt ctaCCTGGTA TCTCGATTAA CTCTGAACAG TTGGCCAGGG 301 CCCAGTGTGT GACTGTGAAA GAGAAGTTAC TTGAGCAAGC AGAGAGCCTT TTGTCGGAGC 361 CTATGGTTCA TGAGCTGGTT CTCTGGATTC AGCAGAATCT CAGGCATATC CTCAGCCAAC 421 CAGAAACTGG CAGTGGCAGT GAAAAGTGTA CTTTTTCAAC AAGCACGACC ATGGATGATG 481 GATTGTGGAT AACTCTTTTG CATTTAGATC ACATGAGAGC AAAGACTAAA TATGTCAAAA 541 TTGTGGAGAA GTGGGCTTCA GATTTAAGGC TGACAGGAAG ACTGATGTTC ATGGGTAAAA 601 TAATACTGAT TTTACTACAG GGAGACAGAA ACAACCTCAA GGTGCCAAAA AGTTTGCCAA 661 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 721 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 781 TATCTTGTGG AAAGGACGAT GTCGGGTTGA TTCAGGCCTC GATACGCGTT AAGTCgacaa 841 tcaacctctg gattacaaaa tttgtgaaag att