Construct: ORF TRCN0000469428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014634.1_s317c1
- Derived from:
- ccsbBroadEn_06696
- DNA Barcode:
- AAATCATTGCACGAGACTATGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCSK1 (5122)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469428
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5122 | PCSK1 | proprotein convertase subti... | NM_000439.5 | 99.9% | 99.7% | 1993C>G;2069G>C |
| 2 | human | 5122 | PCSK1 | proprotein convertase subti... | NM_001177875.1 | 92.4% | 91.4% | (many diffs) |
| 3 | mouse | 18548 | Pcsk1 | proprotein convertase subti... | NM_013628.2 | 88.4% | 92.4% | (many diffs) |
| 4 | mouse | 18548 | Pcsk1 | proprotein convertase subti... | XM_006517153.2 | 88.4% | 92.4% | (many diffs) |
| 5 | mouse | 18548 | Pcsk1 | proprotein convertase subti... | XM_006517154.3 | 88.4% | 92.4% | (many diffs) |
| 6 | mouse | 18548 | Pcsk1 | proprotein convertase subti... | XM_006517155.2 | 88.4% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2328
- ORF length:
- 2259
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcgaaga gcctggagtc tgcagtgcac tgctttcgtc ctcttttgcg 121 cttggtgtgc actgaacagt gcaaaagcga aaaggcaatt tgtcaatgaa tgggcagcgg 181 agatccccgg gggcccggaa gcagcctcgg ccatcgccga ggagctgggc tatgaccttt 241 tgggtcagat tggttcactt gaaaatcact acttattcaa acataaaaac caccccagaa 301 ggtctcgaag gagtgccttt catatcacta agagattatc tgatgatgat cgtgtgatat 361 gggctgaaca acagtatgaa aaagaaagaa gtaaacgttc agctctaagg gactcagcac 421 taaatctctt caatgatccc atgtggaatc agcaatggta cttgcaagat accaggatga 481 cggcagccct gcccaagctg gaccttcatg tgatacctgt ttggcaaaaa ggcattacgg 541 gcaaaggagt tgttatcacc gtactggatg atggtttgga gtggaatcac acggacattt 601 atgccaacta tgatccagag gctagctatg attttaatga taatgaccat gatccatttc 661 cccgatatga tcccacaaac gagaacaaac acgggaccag atgtgcagga gaaattgcca 721 tgcaagcaaa taatcacaaa tgcggggttg gagttgcata caattccaaa gttggaggca 781 taagaatgct ggatggcatt gtgacggatg ctattgaggc cagttcaatt ggattcaatc 841 ctggacacgt ggatatttac agtgcaagct ggggccctaa tgatgatggg aaaactgtgg 901 aggggcctgg ccggctagcc cagaaggctt ttgaatatgg tgtcaaacag gggagacagg 961 ggaaggggtc catcttcgtc tgggcttcgg gaaacggggg gcgtcaggga gataattgtg 1021 actgtgatgg ctacacagac agcatctaca ccatctccat cagcagtgcc tcccagcaag 1081 gcctatcccc ctggtacgct gagaagtgct cctccacact ggccacctct tacagcagcg 1141 gagattacac cgaccagaga atcacgagcg ctgacctgca caatgactgc acggagacgc 1201 acacaggcac ctcggcctct gcacctctgg ctgctggcat cttcgctctg gccctggaag 1261 caaacccaaa tctcacctgg cgagatatgc agcacctggt tgtctggacc tctgagtatg 1321 acccgctggc caataaccct ggatggaaaa agaatggagc aggcttgatg gtgaatagtc 1381 gatttggatt tggcttgcta aatgccaaag ctctggtgga tttagctgac cccaggacct 1441 ggaggagcgt gcctgagaag aaagagtgtg ttgtaaagga caatgacttt gagcccagag 1501 ccctgaaagc taatggagaa gttatcattg aaattccaac aagagcttgt gaaggacaag 1561 aaaatgctat caagtccctg gagcatgtac aatttgaagc aacaattgaa tattcccgaa 1621 gaggagacct tcatgtcaca cttacttctg ctgctggaac tagcactgtg ctcttggctg 1681 aaagagaacg ggatacatct cctaatggct ttaagaattg ggacttcatg tctgttcaca 1741 catggggaga gaaccctata ggtacttgga ctttgagaat tacagacatg tctggaagaa 1801 ttcaaaatga aggaagaatt gtgaactgga agctgatttt gcacgggacc tcttctcagc 1861 cagagcatat gaagcagcct cgtgtgtaca cgTCCTACAA CACTGTTCAG AATGACAGAA 1921 GAGGGGTGGA GAAGATGGTG GATCCAGGGG AGGAGCAGCC CACACAAGAG AACCCTAAGG 1981 AGAACACCCT GGTGTCCAAA AGCCCCAGCA GCAGCAGCGT AGGGGGCCGG AGGGATGAGT 2041 TGGAGGAGGG AGCCCCTTCC GAGGCCATGC TGCGACTCCT GCAAAGTGCT TTCAGTAAAA 2101 ACTCACCGCC AAAGCAATCA CCAAAGAAGT CCCCAACTGC AAAGCTCAAC ATCCCTTATG 2161 AAAACTTCTA CGAAGCCCTG GAAAAGCTGA ACAAACCTTC CCAGCTTAAA GACTCTGAAG 2221 ACAGTCTGTA TAATGACTAT GTTGATGTTT TTTATAACAC TAAACCTTAC AAGCACAGAG 2281 ACGACCGGCT GCTTCAAGCT CTGGTGGACA TTCTGAATGA GGAAAATTTG CCAACTTTCT 2341 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2401 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2461 GTGGAAAGGA CGAAAATCAT TGCACGAGAC TATGGAAACG CGTTAAGTCg acaatcaacc 2521 tctggattac aaaatttgtg aaagatt