Construct: ORF TRCN0000469430
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001738.1_s317c1
- Derived from:
- ccsbBroadEn_05528
- DNA Barcode:
- CAGGCAAAGTTGCAGGTTTTCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TICAM2 (353376)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469430
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 353376 | TICAM2 | toll like receptor adaptor ... | NM_021649.7 | 100% | 100% | |
| 2 | human | 100302736 | TMED7-TICAM2 | TMED7-TICAM2 readthrough | NM_001164468.4 | 56.3% | 53.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 774
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtatcggg aagtctaaaa taaattcctg ccctctttct ctctcttggg 121 gtaaaaggca cagtgtggat acaagtccag gatatcatga gtcagattcc aagaagtctg 181 aagatctatc cttgtgtaat gttgctgagc acagcaatac aacagagggg ccaacaggaa 241 agcaggaggg agctcagagc gtggaagaga tgtttgaaga agaagctgaa gaagaggtgt 301 tcctcaaatt tgtgatattg catgcagaag atgacacaga tgaagccctc agagtccaga 361 atctgctaca agatgacttt GGTATCAAAC CCGGAATAAT CTTTGCTGAG ATGCCATGTG 421 GCAGACAGCA TTTACAGAAT TTAGATGATG CTGTAAATGG GTCTGCATGG ACAATCTTAT 481 TACTGACTGA AAACTTTTTA AGAGATACTT GGTGTAATTT CCAGTTCTAT ACGTCCCTAA 541 TGAACTCCGT TAACAGGCAG CATAAATACA ACTCTGTTAT ACCCATGCGG CCCCTGAACA 601 ATCCCCTTCC CCGAGAAAGG ACTCCCTTTG CCCTCCAAAC CATCAATGCC TTAGAGGAAG 661 AAAGTCGTGG ATTTCCTACA CAAGTAGAAA GAATTTTTCA GGAGTCTGTG TATAAGACAC 721 AACAAACTAT ATGGAAAGAG ACAAGAAATA TGGTACAAAG ACAATTTATT GCCTTGCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAC AGGCAAAGTT GCAGGTTTTC TTAACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att