Construct: ORF TRCN0000469432
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003676.1_s317c1
- Derived from:
- ccsbBroadEn_05410
- DNA Barcode:
- CTCCGCCGAGGCTGCTTGGACTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNPY1 (285888)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469432
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NM_001103176.2 | 100% | 100% | |
| 2 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NM_001369814.1 | 100% | 100% | |
| 3 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NM_001369815.1 | 100% | 100% | |
| 4 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NM_001369816.1 | 100% | 100% | |
| 5 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NM_001369820.1 | 100% | 100% | |
| 6 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NM_001369821.1 | 100% | 100% | |
| 7 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NM_001369813.1 | 50.8% | 50.8% | 1_267del |
| 8 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NM_001369818.1 | 40.3% | 40.3% | 1_408del |
| 9 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163160.1 | 10.7% | 1_498del;775_2571del | |
| 10 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163158.1 | 9.9% | (many diffs) | |
| 11 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163159.1 | 9.4% | (many diffs) | |
| 12 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163157.1 | 9.3% | (many diffs) | |
| 13 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163161.1 | 8.7% | (many diffs) | |
| 14 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163164.1 | 8.6% | 1_1125del;1402_3198del | |
| 15 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163167.1 | 8.6% | (many diffs) | |
| 16 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163163.1 | 8.2% | 1_1125del;1402_3343del | |
| 17 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163166.1 | 8.2% | (many diffs) | |
| 18 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163162.1 | 7.2% | (many diffs) | |
| 19 | human | 285888 | CNPY1 | canopy FGF signaling regula... | NR_163165.1 | 6.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 180
- ORF end:
- 456
- ORF length:
- 276
- Sequence:
-
1 TCGAGCGGCC GCCAGTGTGA TGGAATTCGC CCTTTGAAAC AATGCCGGGA GAGCAAAGCC 61 GCTGAGAGTT TGTCCCGTTA GATCCAGGCG TGAAGGACGG ACTGTACTAC AGGGAGCAGA 121 TCCCCCTAGC CCAGTCGGAG GCGTTCCTAA CGGATCTTTT GGAGAAAGTC TGTGAGCGAA 181 TGAACGACTA CAAGCTTGAG GAAGACCCTG TGACGAAGGA GAGAACTTTC AAGAGATTCG 241 CTCCTAGGAA AGGAGACAAA ATATACCAAG AATTTAAAAA ATTGTATTTT TATTCTGATG 301 CTTACAGACC TTTGAAATTT GCGTGTGAAA CTATAATAGA AGAGTATGAA GATGAAATAT 361 CCTCACTTAT CGCCCAGGAG ACACACTATC TAGCTGACAA GCTGTGCAGT GAAAAATCAG 421 ATCTGTGTGA AACTTCTGCT AATCATACTG AGCTCTTGCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGTTTAT ATATCTTGTG GAAAGGACGA 601 CTCCGCCGAG GCTGCTTGGA CTGAACGCGT TAAGTCgaca atcaacctct ggattacaaa 661 atttgtgaaa gatt