Construct: ORF TRCN0000469434

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013671.1_s317c1
Derived from:
ccsbBroadEn_11471
DNA Barcode:
GTTTCGAAGAAGGCTGTTATGCAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX39A (10212)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469434
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10212 DDX39A DExD-box helicase 39A NM_005804.4 68.8% 55.9% (many diffs)
2 human 10212 DDX39A DExD-box helicase 39A XM_011527620.1 68.8% 55.9% (many diffs)
3 human 10212 DDX39A DExD-box helicase 39A NR_046366.2 61.9% (many diffs)
4 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... NM_197982.3 61.8% 53.8% (many diffs)
5 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_006531332.3 61.8% 53.8% (many diffs)
6 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_017312949.1 57.7% 50.4% (many diffs)
7 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_017312950.1 57.7% 50.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1014
ORF length:
948
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc agaacaggat gtggaaaacg atcttttgga ttacgatgaa gaggaagagc
121cccaggctcc tcaagagagc acaccagctc cccctaagaa agacatcaag ggatcctacg
181tttccatcca cagctctggc ttccgggact ttctgctgaa gccggagctc ctgcgggcca
241tcgtggactg tggctttgag catccttctg aggtccagca tgagtgcatt ccccaggcca
301tcctgggcat ggacgtcctg tgccaggcca agtccgggat gggcaagaca gcggtcttcg
361tgctggccac cctacagcag attgagcctg tcaacggaca ggtgacggtc ctggtcatgt
421gccacacgag ggagctggcc ttccagatca gcaaggaata tgagcgcttt tccaagtaca
481tgcccagcgt caaggtgtct gtgttcttcg gtggtctctc catcaagaag gatgaagaag
541tgttgaagaa gaactgtccc catgtcgtgg tggggacccc gggccgcatc ctggcgctcg
601tgcggaatag gagcttcagc cTAAAGAATG TGAAGCACTT TGTGCTGGAC GAGTGTGACA
661AGATGCTGGA GCAGCTGGAC ATGCGGCGGG ATGTGCAGGA GATCTTCCGC CTGACACCAC
721ACGAGAAGCA GTGCATGATG TTCAGCGCCA CCCTGAGCAA GGACATCCGG CCTGTGTGCA
781GGAAGTTCAT GCAGGATGTG AGTAGGGCGA GAGTGGCAGC TTTGGCCTTC CCCAGGGGCC
841TGGAGCCTGG GGCCCCAGGA CACCCGCACG GCCCACATGG CACGGAGGAT GCTGGGATGC
901TGGGAGCCGG CGGAGCCTCC TTTCCTCCCC TCGGTCTTGT CAAACTGGGA GTCAAGCTTG
961GTCCACACCT GAGTCGGGCC AGAGCTTACA TCCAGCTTTT GAGCGATGTT TACTGCCCAA
1021CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1081ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1141TATCTTGTGG AAAGGACGAG TTTCGAAGAA GGCTGTTATG CATACGCGTT AAGTCgacaa
1201tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)