Construct: ORF TRCN0000469442
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016448.1_s317c1
- Derived from:
- ccsbBroadEn_07877
- DNA Barcode:
- AGGGGGAGCGCTACCATCTCATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPWD1 (23398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469442
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | NM_015342.4 | 99.9% | 100% | 1725A>G |
| 2 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | NM_001278926.1 | 91.6% | 90.5% | 0_1ins127;68_104del;1635A>G |
| 3 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | XM_006714583.4 | 84.7% | 84.5% | (many diffs) |
| 4 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | NM_001278927.2 | 78.2% | 72.6% | 0_1ins247;77_78ins173;1305A>G |
| 5 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | NM_001278929.1 | 75.7% | 75.8% | 0_1ins468;1257A>G |
| 6 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | XM_005248474.4 | 52% | 49.3% | (many diffs) |
| 7 | human | 23398 | PPWD1 | peptidylprolyl isomerase do... | XM_017009286.1 | 49.9% | 50% | 0_1ins969;756A>G |
| 8 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | NM_172807.4 | 90.5% | 97.2% | (many diffs) |
| 9 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XM_006517642.3 | 84.2% | 88.1% | (many diffs) |
| 10 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XR_001780782.1 | 77.3% | (many diffs) | |
| 11 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XM_011244658.2 | 71.6% | 74.3% | (many diffs) |
| 12 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XM_017315513.1 | 69.3% | 74.6% | (many diffs) |
| 13 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XR_873754.2 | 51.6% | (many diffs) | |
| 14 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XM_017315514.1 | 45.7% | 49.2% | (many diffs) |
| 15 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XM_017315515.1 | 45.7% | 49.2% | (many diffs) |
| 16 | mouse | 238831 | Ppwd1 | peptidylprolyl isomerase do... | XR_873753.1 | 44.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2004
- ORF length:
- 1938
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggaaagt ggtagcgatt ttcagcagag acgtagaagg cgccgggacc 121 cggaggaacc ggaaaaaaca gaactcagcg aaagagagct ggcagtagca gtggcggtgt 181 cccaggagaa cgatgaggag aacgaagagc gctgggttgg acctttacct gtggaggcaa 241 cactggccaa gaagaggaaa gtcttagagt ttgaaagagt ctatcttgat aatctcccca 301 gtgcatccat gtatgagcgc agttacatgc atagagatgt tatcacccat gtggtatgca 361 ccaaaacaga ttttattatt actgccagtc atgatggaca tgtcaagttc tggaaaaaaa 421 tagaagaggg aattgaattt gttaaacatt ttcgtagtca cctgggagtt attgagagta 481 ttgcagttag ctctgaggga gcattgttct gttctgtggg tgatgataaa gcaatgaagg 541 tgtttgatgt agtgaacttt gacatgatca acatgctgaa acttggctat tttcctggac 601 agtgtgagtg gatctattgc ccaggggatg caatttcttc agttgctgct tccgaaaaga 661 gtacaggaaa aattttcatt tatgatggcc gaggagataa ccagccactt catatttttg 721 acaaactcca tacatcacct cttactcaga tacggctaaa cccagtttac aaagcagtag 781 tgtcttctga caaatctggg atgattgaat actggactgg gcctcctcat gaatataaat 841 tccccaaaaa tgtgaactgg gaatataaaa ctgacactga tttatatgaa tttgccaagt 901 gtaaggctta tccaaccagc gtatgttttt caccagatgg gaagaaaata gctactattg 961 gttctgatag aaaagttaga attttcagat ttgtaactgg aaaactcatg agagtctttg 1021 atgaatcact aagcatgttt actgaactgc aacagatgag gcaacagtta ccagacatgg 1081 aatttggccg acgaatggct gtagaacgtg agttggagaa ggttgatgct gtaagattaa 1141 ttaatatagt ttttgatgaa actggacact tcgtgctgta tggaacaatg ctgggcatta 1201 aagttataaa tgtagagaca aaccggtgtg tgcggatttt aggcaaacaa gaaaatatta 1261 gagtgatgca attggctttg ttccagggga tagccaaaaa gcatcgtgct gcaactacta 1321 tagaaatgaa agcttctgaa aatcctgttc ttcagaatat tcaagctgac ccaacaatag 1381 tctgtacatc attcaaaaag aatagatttt atatgtttac caaacgagaa ccagaagata 1441 cgaaaagtgc agattctgat cgagatgttt ttaatgagaa accttctaaa gaagaagtca 1501 tggcagctac tcaagctgaa ggacctaaac gagtttcgga cagtgccatt atccacacca 1561 gcatgggaga cattcacacc aaactttttc ctgttgagtg cccTAAGACA GTGGAAAACT 1621 TCTGTGTTCA CAGCAGAAAT GGTTATTATA ATGGGCATAC ATTTCACCGT ATAATTAAGG 1681 GCTTTATGAT TCAGACTGGA GATCCAACAG GTACTGGTAT GGGAGGAGAA AGCATATGGG 1741 GAGGAGAATT TGAAGATGAA TTTCATTCAA CATTACGACA TGACAGGCCG TACACACTCA 1801 GCATGGCTAA CGCGGGATCA AATACTAATG GATCCCAGTT TTTCATAACG GTAGTACCAA 1861 CGCCTTGGCT TGATAATAAG CATACAGTAT TTGGACGAGT GACTAAAGGA ATGGAAGTTG 1921 TACAGAGGAT CTCCAACGTC AAAGTCAATC CCAAAACAGA TAAGCCCTAT GAGGATGTCA 1981 GCATCATAAA TATTACTGTC AAGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2041 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2101 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GGGGGAGCGC 2161 TACCATCTCA TTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2221 att