Construct: ORF TRCN0000469445

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002467.1_s317c1
Derived from:
ccsbBroadEn_06704
DNA Barcode:
CCTCGGCCAACCGAACAACCATAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDE4D (5144)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469445
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5144 PDE4D phosphodiesterase 4D NM_001197223.2 99.8% 99.8% 656C>T;1014G>A
2 human 5144 PDE4D phosphodiesterase 4D NM_001197221.2 97.3% 96.9% (many diffs)
3 human 5144 PDE4D phosphodiesterase 4D NM_001364603.1 97.3% 96.9% (many diffs)
4 human 5144 PDE4D phosphodiesterase 4D NM_001349243.2 92.4% 91.6% (many diffs)
5 human 5144 PDE4D phosphodiesterase 4D NM_001364604.1 92.4% 91.6% (many diffs)
6 human 5144 PDE4D phosphodiesterase 4D NM_001197222.2 87.4% 86.6% (many diffs)
7 human 5144 PDE4D phosphodiesterase 4D NM_006203.4 75.9% 75.3% (many diffs)
8 human 5144 PDE4D phosphodiesterase 4D NM_001197220.2 75.3% 74.6% (many diffs)
9 human 5144 PDE4D phosphodiesterase 4D NM_001197219.2 74.4% 73.7% (many diffs)
10 human 5144 PDE4D phosphodiesterase 4D NM_001349242.1 73.1% 72.5% (many diffs)
11 human 5144 PDE4D phosphodiesterase 4D NM_001349241.2 69.3% 68.6% (many diffs)
12 human 5144 PDE4D phosphodiesterase 4D XM_017009567.1 68.8% 68.2% (many diffs)
13 human 5144 PDE4D phosphodiesterase 4D NM_001197218.2 68.6% 68% (many diffs)
14 human 5144 PDE4D phosphodiesterase 4D NM_001165899.2 68.3% 67.7% (many diffs)
15 human 5144 PDE4D phosphodiesterase 4D NM_001364599.1 68.3% 67.7% (many diffs)
16 human 5144 PDE4D phosphodiesterase 4D XM_011543471.2 68.3% 67.7% (many diffs)
17 human 5144 PDE4D phosphodiesterase 4D XM_011543473.1 68.3% 67.7% (many diffs)
18 human 5144 PDE4D phosphodiesterase 4D XM_017009566.1 68.3% 67.7% (many diffs)
19 human 5144 PDE4D phosphodiesterase 4D XM_024446112.1 68.3% 67.7% (many diffs)
20 human 5144 PDE4D phosphodiesterase 4D XM_011543469.1 64.1% 63.6% (many diffs)
21 human 5144 PDE4D phosphodiesterase 4D XM_011543470.2 64.1% 63.6% (many diffs)
22 human 5144 PDE4D phosphodiesterase 4D XM_017009565.1 64.1% 63.6% (many diffs)
23 human 5144 PDE4D phosphodiesterase 4D XM_024446110.1 64.1% 63.6% (many diffs)
24 human 5144 PDE4D phosphodiesterase 4D NM_001104631.2 63.2% 62.6% (many diffs)
25 mouse 238871 Pde4d phosphodiesterase 4D, cAMP ... XM_006517648.3 89% 97.8% (many diffs)
26 mouse 238871 Pde4d phosphodiesterase 4D, cAMP ... XM_006517649.3 86.6% 94.9% (many diffs)
27 mouse 238871 Pde4d phosphodiesterase 4D, cAMP ... XM_006517647.2 67.1% 73.1% (many diffs)
28 mouse 238871 Pde4d phosphodiesterase 4D, cAMP ... XM_006517646.3 66.3% 72.3% (many diffs)
29 mouse 238871 Pde4d phosphodiesterase 4D, cAMP ... XM_006517645.3 64.7% 70.5% (many diffs)
30 mouse 238871 Pde4d phosphodiesterase 4D, cAMP ... XM_006517644.2 61.1% 66.6% (many diffs)
31 mouse 238871 Pde4d phosphodiesterase 4D, cAMP ... NM_011056.3 60.9% 66.4% (many diffs)
32 mouse 238871 Pde4d phosphodiesterase 4D, cAMP ... XM_006517643.1 56.7% 61.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1620
ORF length:
1554
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc tgaagcaaac tatttactgt cagtgtcttg gggctacata aagtttaaaa
121ggatgcttaa tcgggagctc acccatctct ctgaaatgag tcggtctgga aatcaagtgt
181cagagtttat atcaaacaca ttcttagata agcaacatga agtggaaatt ccttctccaa
241ctcagaagga aaaggagaaa aagaaaagac caatgtctca gatcagtgga gtcaagaaat
301tgatgcacag ctctagtctg actaattcaa gtatcccaag gtttggagtt aaaactgaac
361aagaagatgt ccttgccaag gaactagaag atgtgaacaa atggggtctt catgttttca
421gaatagcaga gttgtctggt aaccggccct tgactgttat catgcacacc atttttcagg
481aacgggattt attaaaaaca tttaaaattc cagtagatac tttaattaca tatcttatga
541ctctcgaaga ccattaccat gctgatgtgg cctatcacaa caatatccat gctgcagatg
601ttgtccagtc tactcatgtg ctattatcta cacctgcttt ggaggctgtg tttacagatt
661tggagattct tgcagcaatt tttgccagtg caatacatga tgtagatcat cctggtgtgt
721tcaatcaatt tctgatcaat acaaactctg aacttgcctt gatgtacaat gattcctcag
781tcttagagaa ccatcatttg gctgtgggct ttaaattgct tcaggaagaa aactgtgaca
841ttttccagaa tttgaccaaa aaacaaagac aatctttaag gaaaatggtc attgacatcg
901tacttgcaac agatatgtca aaacacatga atctactggc tgatttgaag actatggttg
961aaactaagaa agtgacaagc tctggagttc ttcttcttga taattattcc gataggattc
1021aggttcttca gaatatggtg cactgtgcag atctgagcaa cccaacaaag cctctccaac
1081tgtaccgcca gtggacggac cggataatgg aggagttctt ccgccaagga gaccgagaga
1141gggaacgtgg catggagata agccccatgt gtgacaagca caatgcttcc gtggaaaaat
1201cacaggtggg cttcatagac tatattgttc aTCCCCTCTG GGAGACATGG GCAGACCTCG
1261TCCACCCTGA CGCCCAGGAT ATTTTGGACA CTTTGGAGGA CAATCGTGAA TGGTACCAGA
1321GCACAATCCC TCAGAGCCCC TCTCCTGCAC CTGATGACCC AGAGGAGGGC CGGCAGGGTC
1381AAACTGAGAA ATTCCAGTTT GAACTAACTT TAGAGGAAGA TGGTGAGTCA GACACGGAAA
1441AGGACAGTGG CAGTCAAGTG GAAGAAGACA CTAGCTGCAG TGACTCCAAG ACTCTTTGTA
1501CTCAAGACTC AGAGTCTACT GAAATTCCCC TTGATGAACA GGTTGAAGAG GAGGCAGTAG
1561GGGAAGAAGA GGAAAGCCAG CCTGAAGCCT GTGTCATAGA TGATCGTTCT CCTGACACGT
1621ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1681GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1741TTTATATATC TTGTGGAAAG GACGACCTCG GCCAACCGAA CAACCATACA CGCGTTAAGT
1801Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

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