Construct: ORF TRCN0000469462
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018919.3_s317c1
- Derived from:
- ccsbBroadEn_14757
- DNA Barcode:
- TCACCCTAAAAATAAACTTTCACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PIK3C2G (5288)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469462
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | NM_004570.5 | 99.5% | 80.6% | (many diffs) |
| 2 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | NM_001288772.2 | 96.8% | 78.3% | (many diffs) |
| 3 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_017019474.2 | 96.5% | 80.2% | (many diffs) |
| 4 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_011520696.2 | 94.4% | 78.9% | (many diffs) |
| 5 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_017019470.1 | 93.9% | 78% | (many diffs) |
| 6 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_017019471.1 | 93.9% | 78% | (many diffs) |
| 7 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_017019472.1 | 93.9% | 78% | (many diffs) |
| 8 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_017019473.2 | 93.9% | 78% | (many diffs) |
| 9 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_011520697.2 | 93.7% | 80.7% | (many diffs) |
| 10 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_017019476.1 | 93.5% | 77.2% | (many diffs) |
| 11 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_017019475.1 | 93.4% | 81% | (many diffs) |
| 12 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | NM_001288774.1 | 82% | 63.2% | (many diffs) |
| 13 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_017019477.1 | 79.3% | 62.9% | (many diffs) |
| 14 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_017019479.1 | 53.5% | 37.4% | (many diffs) |
| 15 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_011520700.2 | 53.1% | 62.7% | (many diffs) |
| 16 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_011520701.2 | 53.1% | 62.7% | (many diffs) |
| 17 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XM_017019478.2 | 53.1% | 62.7% | (many diffs) |
| 18 | human | 5288 | PIK3C2G | phosphatidylinositol-4-phos... | XR_931307.2 | 50.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3669
- ORF length:
- 3600
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcatattct tggcaaacgg atccaaatcc taatgaatca cacgaaaagc 121 agtatgaaca ccaagaattt ctctttgtaa atcaacccca ttcttctagc caagtcagtc 181 tgggttttga tcagatagta gatgagatca gtggcaaaat tccacactac gagagtgaaa 241 ttgatgaaaa cacctttttt gtgcccactg caccaaaatg ggactcaaca gggcattcat 301 taaatgaagc acaccaaata tccttgaatg aattcacttc taaaagccgt gaactctcct 361 ggcatcaagt tagcaaagca ccagcaattg gttttagtcc ttctgtgtta ccaaaacctc 421 aaaatacgaa taaagaatgc tcctggggaa gcataggaaa acatcatggt gctgatgatt 481 ccagattcag tattttagct ctatcattca caagtttgga taaaattaat ctagagaaag 541 aattagaaaa tgaaaatcat aactaccata taggatttga aagtagcatt cctccaacaa 601 attcatcctt ctcaagtgac ttcatgccga aagaagagaa taaaaggagt ggacatgtga 661 acattgtgga accatctttg atgcttttga aaggctctct tcaacccgga atgtgggaaa 721 gtacatggca gaagaatata gagtcaatat gttgttccat tcagctagtg gaagtacctc 781 aagcagcata cgagtctggc ctctttttgc acaaagtaaa aaaaatcaga gaaagatatc 841 atgcagctga tgttaatttc aattctggga agatctggag cactactaca gcatttccgt 901 atcagctctt ttctaagacc aagtttaata tacatatttt tattgataac tcaacacaac 961 ctcttcattt tatgccatgt gctaattatc ttgtcaaaga tctaattgca gaaattctgc 1021 atttttgcac aaatgaccag ctactcccca aagatcatat tctaagtgta tgtggctctg 1081 aagaattttt acaaaacgac cactgtttgg ggagccacaa aatgtttcaa aaagataaat 1141 ctgttattca gctccacctg cagaaaagta gggaagctcc aggaaagcta tctcgaaagc 1201 atgaagagga ccacagtcag ttttatctga atcaacttct agaatttatg catatttgga 1261 aagtatccag acaatgtctc ttaacactca tcagaaaata tgacttccac ctgaaatacc 1321 tattgaaaac ccaggaaaac gtgtataata ttattgaaga agttaaaaaa atatgcagtg 1381 ttctagggtg tgtggaaacc aaacaaatta cagatgcagt aaatgaacta agtctaattc 1441 ttcagagaaa aggagagaat ttttatcaaa gttcagagac ttcagcaaaa ggcttgatag 1501 agaaggtaac aactgaacta tccacatcca tctaccagct aatcaatgtc tactgtaaca 1561 gcttttatgc agattttcag cctgtaaatg tacctagatg cacttcctat ctaaatcccg 1621 ggcttccttc ccacctcagc ttcacagtgt atgcagcaca caacattcca gaaacctggg 1681 tgcacaggat caattttccc cttgaaataa agtcacttcc aagggaatcc atgctcactg 1741 taaaactgtt tgggattgcc tgtgcaacca acaatgcaaa tttactggcg tggacttgtc 1801 ttccactgtt tccaaaagaa aaatccattc tcgggtctat gctgttcagc atgacattac 1861 agagtgagcc tcccgtagaa atgataactc caggagtgtg ggatgtaagt cagccatccc 1921 cggtgaccct gcagattgat tttccagcta ctgggtggga gtatatgaaa cctgattctg 1981 aagagaatag aagtaatctt gaagagccac taaaggagtg tataaaacat attgccagac 2041 tttcacagaa acagactccc ctactactct ctgaagaaaa gaaaagatat ttatggtttt 2101 atcgcttcta ctgcaataat gaaaactgct cccttccttt agtcctgggt agtgcccctg 2161 gatgggatga aaggactgtt tcagaaatgc ataccatttt gagaagatgg acattttctc 2221 aacctttaga ggctcttggg cttttgactt ccagttttcc agatcaagaa attcgtaaag 2281 tggcagttca acaattagac aacctcttga atgatgaact actggaatat ctcccacagc 2341 tagttcaggc tgtcaagttt gaatggaacc ttgagagtcc tttagtgcaa cttctactcc 2401 accgctcctt gcagagcatc caggttgccc atcgtcttta ctggctgcta aaaaatgcag 2461 aaaatgaagc ttattttaaa agctggtatc agaagctact agctgctctc caattctgtg 2521 caggtaaagc cttgaatgat gagttttcca aggagcagaa acttatcaaa attctgggag 2581 atattgggga aagagtcaag tctgccagtg accatcaaag acaggaggta ctgaagaaag 2641 aaattggcag actagaagag ttctttcaag atgtaaatac ttgtcatctt cctctgaacc 2701 ctgccctatg tataaaaggg attgatcacg atgcatgttc atattttaca tctaatgctt 2761 tgccattgaa gattactttc atcaatgcta atccgatggg caaaaacatc agcattattt 2821 ttaaggctgg agatgatctt cgtcaggata tgcttgttct gcagcttatt caagtgatgg 2881 acaatatttg gctgcaggaa ggcttggata tgcaaatgat catttataga tgtctatcca 2941 caggaaaaga ccaaggattg gtgcagatgg tacctgatgc tgtgacccta gcaaagattc 3001 atcgccattc tggactgata ggaccattga aagaaaatac aattaaaaag tggttcagtc 3061 agcacaacca cttaaaggca gattatgaaa aggccttgag gaactttttc tactcctgtg 3121 ctggctggtg tgtggtaaca ttcatcctgg gagtatgtga ccgtcacaat gataatatca 3181 tgctgacaaa gtcgggccac atgtttcata ttgactttgg aaaattctta ggtcatgcac 3241 aaacatttgg agggataaaa agggaccgag ctcctttcat ttttacttca gagatggaat 3301 actttattac agagggtggg aaaaacccac agcattttca agattttgtg gaactttgct 3361 gtcgtgctta taatattatc agaaagcaca gccaactgct cttgaacctg ctggaaatga 3421 tgctgtatgc aggactgcct gagctaagtg gaattcaaga cctgaaatat gtgtataata 3481 atcttcgtcc acaagacaca gacctggaag caacaagtca ttttaccaag aaaataaagg 3541 aaagtctgga gtgtttccct gttaaattga ataacttgat ccaccccact tgcacaaaag 3601 tcaaccataa gccctgccaa atttactcca cagacttttc ttcaagaatc ctgtttgctg 3661 agtacaacta ggtcgatgaa aagagcaaca attttagggt tcagcaagaa atccagtaat 3721 ctgtatctga tccaggtgac acacagcaac aacgaaacaa gcctgacaga aaaatcattt 3781 gagcagtttt caaaacttca cagccaactt cagaagcagt ttgcatcact gactctccca 3841 gagtttcctc attggtggca cctacctttt acaaattcag atcacagaag attcagagat 3901 ctaaatcatt acatggaaca gatattaaat gtatcacatg aagttacaaa cagtgattgt 3961 gtacttagct ttttcctctc tgaggctgtg caacaaacag ttgaagaatc atcacctgtg 4021 tacctaggtg agaagtttcc agacaagaag ccTAAGGTGC AGTTAGTCAT ATCCTACGAG 4081 GATGTGAAGC TGACCATACT AGTGAAACAC ATGAAAAACA TTCATCTCCC AGATGGCTCT 4141 GCGCCCAGTG CACATGTTGA ATTTTATCTT TTACCATATC CCAGTGAAGT TCGTAGGAGG 4201 AAAACAAAAT CTGTTCCAAA ATGTACGGAC CCCACTTACA ATGAAATTGT AGTATATGAT 4261 GAAGTCACAG AGCTCCAAGG ACATGTCTTA ATGCTTATTG TGAAGAGTAA AACTGTATTT 4321 GTGGGAGCAA TTAACATCCG ACTCTGTAGT GTCCCACTCG ATAAAGAAAA ATGGTATCCA 4381 TTAGGAAACA GTATAATTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT 4441 ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA 4501 AGTATTTCGA TTTCTTGGGC TTTATATATC TTGTGGAAAG GACGATCACC CTAAAAATAA 4561 ACTTTCACCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt