Construct: ORF TRCN0000469471
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001883.1_s317c1
- Derived from:
- ccsbBroadEn_12394
- DNA Barcode:
- ATTTTATTATCGAGGAAGTGACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SHTN1 (57698)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469471
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57698 | SHTN1 | shootin 1 | NM_001258298.1 | 21.8% | 21.8% | 1_1338del |
| 2 | human | 57698 | SHTN1 | shootin 1 | NM_001127211.3 | 19.8% | 19.8% | 1_1518del |
| 3 | human | 57698 | SHTN1 | shootin 1 | XM_005270002.4 | 9.2% | 8.5% | (many diffs) |
| 4 | human | 57698 | SHTN1 | shootin 1 | NM_001258300.1 | 9% | 9.1% | 1_1338del;1494_1494delAins220 |
| 5 | human | 57698 | SHTN1 | shootin 1 | NM_001258299.1 | 8.1% | 8.2% | 1_1518del;1674_1674delAins220 |
| 6 | mouse | 71653 | Shtn1 | shootin 1 | NM_001114312.1 | 17.4% | 15.5% | (many diffs) |
| 7 | mouse | 71653 | Shtn1 | shootin 1 | XR_386512.3 | 7.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc agtgttgggt TCTGTATCCA GTGTAACAAA AACAGCCTTG AACAAGAAAA 121 CTCTGGAGGC AGAATTCAAC AGCCCGTCCC CCCCAACACC TGAGCCAGGT GAAGGGCCCC 181 GTAAATTGGA AGGATGCACA AGTTCCAAGG TTACGTTTCA GCCTCCCAGT AGCATTGGAT 241 GCAGGAAAAA ATACATTGAC GGTGAAAAAC AAGCCGAACC AGTTGTAGTT TTAGATCCTG 301 TTTCTACACA TGAACCCCAA ACCAAAGACC AGGTTGCTGA AAAAGATCCA ACTCAACACA 361 AGGAGGATGA AGGCGAAATT CAACCAGAAA ACAAAGAAGA CAGCATTGAA AACGTGAGAG 421 AGACAGACAG CTCCAACTGC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATTT TATTATCGAG 601 GAAGTGACGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt