Transcript: Human NM_001127211.3

Homo sapiens shootin 1 (SHTN1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
SHTN1 (57698)
Length:
6871
CDS:
109..2004

Additional Resources:

NCBI RefSeq record:
NM_001127211.3
NBCI Gene record:
SHTN1 (57698)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001127211.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000062748 GCACATGATTAGTGTTGTTAT pLKO.1 2050 3UTR 100% 13.200 18.480 N SHTN1 n/a
2 TRCN0000286930 GCACATGATTAGTGTTGTTAT pLKO_005 2050 3UTR 100% 13.200 18.480 N SHTN1 n/a
3 TRCN0000294282 GAGCAAGCAATAGGCGAATAT pLKO_005 160 CDS 100% 13.200 10.560 N SHTN1 n/a
4 TRCN0000062751 CGAAAGAAAGCAGAGTCATTT pLKO.1 790 CDS 100% 13.200 9.240 N SHTN1 n/a
5 TRCN0000286928 CGAAAGAAAGCAGAGTCATTT pLKO_005 790 CDS 100% 13.200 9.240 N SHTN1 n/a
6 TRCN0000294283 GAACGAGATGAAGCCGTTAAA pLKO_005 238 CDS 100% 13.200 9.240 N SHTN1 n/a
7 TRCN0000062749 CGAAGACAAAGCCAGAATCTT pLKO.1 1403 CDS 100% 5.625 3.938 N SHTN1 n/a
8 TRCN0000062752 CGAGATCAAATTGTATCTGTT pLKO.1 550 CDS 100% 4.950 3.465 N SHTN1 n/a
9 TRCN0000062750 GCAGCTCATTACCAGTCTGAA pLKO.1 138 CDS 100% 4.950 2.970 N SHTN1 n/a
10 TRCN0000286929 GCAGCTCATTACCAGTCTGAA pLKO_005 138 CDS 100% 4.950 2.970 N SHTN1 n/a
11 TRCN0000071709 GCACAAGAGATGTTCATTGAA pLKO.1 811 CDS 100% 5.625 3.938 N Shtn1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001127211.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12395 pDONR223 100% 88.3% 88.4% None 1674_1893delinsA n/a
2 ccsbBroad304_12395 pLX_304 0% 88.3% 88.4% V5 1674_1893delinsA n/a
3 TRCN0000492086 TAAAATCTGCCCGTAGTTAATCAG pLX_317 26.8% 88.3% 88.4% V5 1674_1893delinsA n/a
4 ccsbBroadEn_12394 pDONR223 100% 19.8% 19.8% None 1_1518del n/a
5 ccsbBroad304_12394 pLX_304 0% 19.8% 19.8% V5 1_1518del n/a
6 TRCN0000469471 ATTTTATTATCGAGGAAGTGACGT pLX_317 93.9% 19.8% 19.8% V5 1_1518del n/a
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