Construct: ORF TRCN0000469482
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014380.1_s317c1
- Derived from:
- ccsbBroadEn_00865
- DNA Barcode:
- GGTGATCGACTTGTCTACCCGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ILF2 (3608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469482
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3608 | ILF2 | interleukin enhancer bindin... | NM_004515.4 | 100% | 100% | |
| 2 | human | 3608 | ILF2 | interleukin enhancer bindin... | NM_001267809.1 | 90.2% | 90.2% | 0_1ins114 |
| 3 | mouse | 67781 | Ilf2 | interleukin enhancer bindin... | NM_026374.3 | 91.5% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gggtgacaga ggccgtggtc gtggtgggcg ctttggttcc agaggaggcc 121 caggaggagg gttcaggccc tttgtaccac atatcccatt tgacttctat ttgtgtgaaa 181 tggcctttcc ccgggtcaag ccagcacctg atgaaacttc cttcagtgag gccttgctga 241 agaggaatca ggacctggct cccaattctg ctgaacaggc atctatcctt tctctggtga 301 caaaaataaa caatgtgatt gataatctga ttgtggctcc agggacattt gaagtgcaaa 361 ttgaagaagt tcgacaggtg ggatcctata aaaaggggac aatgactaca ggacacaatg 421 tggctgacct ggtggtgata ctcaagattc tgccaacgtt ggaagctgtt gctgccctgg 481 ggaacaaagt cgtggaaagc ctaagagcac aggatccttc tgaagtttta accatgctga 541 ccaacgaaac tggctttgaa atcagttctt ctgatgctac agtgaagatt ctcattacaa 601 cagtgccacc caatcttcga aaactggatc cagaactcca tttggatatc aaagtattgc 661 agagtgcctt agcagccatc cgacatgccc gctggttcga ggaaaatgct tctcagtcca 721 cagttaaagt tctcatcaga ctactgaagg acttgaggat tcgttttcct ggctttgagc 781 ccctcacacc ctggatcctt gacctactag gccattatgc tgtgatgaac aaccccacca 841 gacagccttt ggccctaaac gttgcataca ggcgctgctt gcagatTCTG GCTGCAGGAC 901 TGTTCCTGCC AGGTTCAGTG GGTATCACTG ACCCCTGTGA GAGTGGCAAC TTTAGAGTAC 961 ACACAGTCAT GACCCTAGAA CAGCAGGACA TGGTCTGCTA TACAGCTCAG ACTCTCGTCC 1021 GAATCCTCTC ACATGGTGGC TTTAGGAAGA TCCTTGGCCA GGAGGGTGAT GCCAGCTATC 1081 TTGCTTCTGA AATATCTACC TGGGATGGAG TGATAGTAAC ACCTTCAGAA AAGGCTTATG 1141 AGAAGCCACC AGAGAAGAAG GAAGGAGAGG AAGAAGAGGA GAATACAGAA GAACCACCTC 1201 AAGGAGAGGA AGAAGAAAGC ATGGAAACTC AGGAGTGCCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 AGGTGATCGA CTTGTCTACC CGGCAACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt