Construct: ORF TRCN0000469512
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008206.1_s317c1
- Derived from:
- ccsbBroadEn_12830
- DNA Barcode:
- CTCAGTCAGACTCGTTTTTATGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAXC (84553)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469512
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84553 | FAXC | failed axon connections hom... | XM_006715582.2 | 66.8% | 66.8% | 1_192del |
| 2 | human | 84553 | FAXC | failed axon connections hom... | NM_001346530.2 | 44.7% | 44.7% | 1_477del |
| 3 | human | 84553 | FAXC | failed axon connections hom... | NM_001346533.1 | 44.7% | 44.7% | 1_477del |
| 4 | human | 84553 | FAXC | failed axon connections hom... | NM_001346531.2 | 36.2% | 36.2% | 1_681del |
| 5 | human | 84553 | FAXC | failed axon connections hom... | NM_001346532.1 | 36.2% | 36.2% | 1_681del |
| 6 | human | 84553 | FAXC | failed axon connections hom... | NM_032511.4 | 31.5% | 31.5% | 1_840del |
| 7 | human | 84553 | FAXC | failed axon connections hom... | NR_144463.2 | 3.5% | 1_513del;901_10899del | |
| 8 | human | 84553 | FAXC | failed axon connections hom... | NR_144464.1 | 3.4% | 1_732del;1120_11125del | |
| 9 | mouse | 76132 | Faxc | failed axon connections hom... | XM_006538340.3 | 40.6% | 44% | (many diffs) |
| 10 | mouse | 76132 | Faxc | failed axon connections hom... | NM_175234.4 | 28.6% | 31% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 453
- ORF length:
- 387
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcccaagctt tccactcttg acgccactgt ctttggacac ttggcacagg 121 caatgtggac cTTACCAGGG ACAAGACCCG AACGGCTGAT CAAAGGTGAG CTGATCAACC 181 TTGCCATGTA CTGTGAGAGG ATAAGGAGGA AATTTTGGCC AGAGTGGCAC CACGATGATG 241 ACAATACCAT CTATGAGTCT GAGGAGAGCA GCGAAGGCAG CAAAACCCAC ACCCCGCTGC 301 TGGATTTTAG CTTTTACTCA AGGACAGAGA CCTTTGAAGA TGAGGGAGCA GAAAACAGTT 361 TTTCCAGAAC CCCAGACACA GATTTTACTG GACACTCACT CTTTGATTCG GATGTGGACA 421 TGGATGACTA TACAGACCAC GAACAGTGCA AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 481 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 541 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 601 CAGTCAGACT CGTTTTTATG GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 661 ttgtgaaaga tt