Construct: ORF TRCN0000469517

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005550.2_s317c1
Derived from:
ccsbBroadEn_03118
DNA Barcode:
GTTGCGTTATAGTGTGGGCTCCCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NRBP1 (29959)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469517
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29959 NRBP1 nuclear receptor binding pr... NM_001321357.1 100% 100%
2 human 29959 NRBP1 nuclear receptor binding pr... NM_013392.4 100% 100%
3 human 29959 NRBP1 nuclear receptor binding pr... NM_001321362.2 99.8% 99.8% 743_744insAGA
4 human 29959 NRBP1 nuclear receptor binding pr... NM_001321358.2 98.5% 98.5% 660_683del
5 human 29959 NRBP1 nuclear receptor binding pr... NM_001321359.2 98.5% 98.5% 660_683del
6 human 29959 NRBP1 nuclear receptor binding pr... NM_001321361.2 98.3% 98.3% 660_683del;926_927insGGA
7 human 29959 NRBP1 nuclear receptor binding pr... NM_001321363.2 94% 94% 338_339ins96
8 mouse 192292 Nrbp1 nuclear receptor binding pr... NM_147201.2 93.2% 98.3% (many diffs)
9 mouse 192292 Nrbp1 nuclear receptor binding pr... NM_001347326.1 91.8% 96.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1671
ORF length:
1605
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc ggagggggag tcccagacag tacttagcag tggctcagac ccaaaggtag
121aatcctcatc ttcagctcct ggcctgacat cagtgtcacc tcctgtgacc tccacaacct
181cagctgcttc cccagaggaa gaagaagaaa gtgaagatga gtctgagatt ttggaagagt
241cgccctgtgg gcgctggcag aagaggcgag aagaggtgaa tcaacggaat gtaccaggta
301ttgacagtgc atacctggcc atggatacag aggaaggtgt agaggttgtg tggaatgagg
361tacagttctc tgaacgcaag aactacaagc tgcaggagga aaaggttcgt gctgtgtttg
421ataatctgat tcaattggag catcttaaca ttgttaagtt tcacaaatat tgggctgaca
481ttaaagagaa caaggccagg gtcattttta tcacagaata catgtcatct gggagtctga
541agcaatttct gaagaagacc aaaaagaacc acaagacgat gaatgaaaag gcatggaagc
601gttggtgcac acaaatcctc tctgccctaa gctacctgca ctcctgtgac ccccccatca
661tccatgggaa cctgacctgt gacaccatct tcatccagca caacggactc atcaagattg
721gctctgtggc tcctgacact atcaacaatc atgtgaagac ttgtcgagaa gagcagaaga
781atctacactt ctttgcacca gagtatggag aagtcactaa tgtgacaaca gcagtggaca
841tctactcctt tggcatgtgt gcactggaga tggcagtgct ggagattcag ggcaatggag
901agtcctcata tgtgccacag gaagccatca gcagtgccat ccagcttcta gaagacccat
961tacagaggga gttcattcaa aagtgcctgc agtctgagcc tgctcgcaga ccaacagcca
1021gagaacttct gttccaccca gcattgtttg aagtgccctc gctcaaactc cttgcggccc
1081actgcattgt gggacaccaa cacatgatcc cagagaacgc tctagaggag atcaccaaaa
1141acatggatac tagtgccgta ctggctgaaa tccctgcagg accaggaaga gaaccagttc
1201agactttgta ctctcagtca ccagctctgg aattagataa attccttgaa gatgtcagga
1261atgggatcta tcctctgaca gcctttgggc tgcctcggcc ccagcagcca cagcaggagg
1321aggtgacatc acctgtcgtg cccccctctg tcaagacTCC GACACCTGAA CCAGCTGAGG
1381TGGAGACTCG CAAGGTGGTG CTGATGCAGT GCAACATTGA GTCGGTGGAG GAGGGAGTCA
1441AACACCACCT GACACTTCTG CTGAAGTTGG AGGACAAACT GAACCGGCAC CTGAGCTGTG
1501ACCTGATGCC AAATGAGAAT ATCCCCGAGT TGGCGGCTGA GCTGGTGCAG CTGGGCTTCA
1561TTAGTGAGGC TGACCAGAGC CGGTTGACTT CTCTGCTAGA AGAGACCTTG AACAAGTTCA
1621ATTTTGCCAG GAACAGTACC CTCAACTCAG CCGCTGTCAC CGTCTCCTCT TACCCAACTT
1681TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1741CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1801CTTGTGGAAA GGACGAGTTG CGTTATAGTG TGGGCTCCCA ACGCGTTAAG TCgacaatca
1861acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)