Construct: ORF TRCN0000469519
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012784.1_s317c1
- Derived from:
- ccsbBroadEn_14325
- DNA Barcode:
- GATAAATCTCCATATCTAGACTGC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM17 (84991)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469519
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84991 | RBM17 | RNA binding motif protein 17 | NM_001145547.1 | 99.9% | 99% | 1191delG |
| 2 | human | 84991 | RBM17 | RNA binding motif protein 17 | NM_032905.5 | 99.9% | 99% | 1191delG |
| 3 | human | 84991 | RBM17 | RNA binding motif protein 17 | XR_001747246.2 | 35.9% | (many diffs) | |
| 4 | mouse | 76938 | Rbm17 | RNA binding motif protein 17 | NM_152824.1 | 89.4% | 97.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1269
- ORF length:
- 1203
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cctgtacgat gacctaggag tggagaccag tgactcaaaa acagaaggct 121 ggtccaaaaa cttcaaactt ctgcagtctc agcttcaggt gaagaaggca gctctcactc 181 aggcaaagag ccaaaggacg aaacaaagta cagtcctcgc cccagtcatt gacctgaagc 241 gaggtggctc ctcagatgac cggcaaattg tggacactcc accgcatgta gcagctgggc 301 tgaaggatcc tgttcccagt gggttttctg caggggaagt tctgattccc ttagctgacg 361 aatatgaccc tatgtttcct aatgattatg agaaagtagt gaagcgccaa agagaggaac 421 gacagagaca gcgggagctg gaaagacaaa aggaaataga agaaagggaa aaaaggcgta 481 aagacagaca tgaagcaagt gggtttgcaa ggagaccaga tccagattct gatgaagatg 541 aagattatga gcgagagagg aggaaaagaa gtatgggcgg agctgccatt gccccaccca 601 cttctctggt agagaaagac aaagagttac cccgagattt tccttatgaa gaggactcaa 661 gacctcgatc acagtcttcc aaagcagcca ttcctccccc agtgtacgag gaacaagaca 721 gaccgagatc tccaaccgga cctagcaact ccttcctcgc taacatgggg ggcacggtgg 781 cgcacaagat catgcagaag tacggcttcc gggagggcca gggtctgggg aagcatgagc 841 agggcctgag cactgccttg tcagtggaga agaccagcaa gcgtggcggc aagaTCATCG 901 TGGGCGACGC CACAGAGAAA GATGCATCCA AGAAGTCAGA TTCAAATCCG CTGACTGAAA 961 TACTTAAGTG TCCTACTAAA GTGGTCTTAC TAAGGAACAT GGTTGGTGCG GGAGAGGTGG 1021 ATGAAGACTT GGAAGTTGAA ACCAAGGAAG AATGTGAAAA ATATGGCAAA GTTGGAAAAT 1081 GTGTGATATT TGAAATTCCT GGTGCCCCTG ATGATGAAGC AGTACGGATA TTTTTAGAAT 1141 TTGAGAGAGT TGAATCAGCA ATTAAAGCGG TTGTTGACTT GAATGGGAGG TATTTTGGTG 1201 GACGGGTGGT AAAAGCATGT TTCTACAATT TGGACAAATT CAGGGTCTTG GATTTGCAGA 1261 ACAAGTTTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 1321 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 1381 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGATAAAT CTCCATATCT AGACTGCACG 1441 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt