Construct: ORF TRCN0000469530
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015273.1_s317c1
- Derived from:
- ccsbBroadEn_03840
- DNA Barcode:
- CGCTACACTAATTACGTTCGAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VAT1L (57687)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469530
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57687 | VAT1L | vesicle amine transport 1 like | NM_020927.3 | 99.9% | 100% | 468C>G |
| 2 | mouse | 270097 | Vat1l | vesicle amine transport pro... | NM_173016.3 | 88.7% | 98% | (many diffs) |
| 3 | mouse | 270097 | Vat1l | vesicle amine transport pro... | XM_011248394.1 | 76.3% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1323
- ORF length:
- 1257
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caaggaaggc gtggagaagg cggaggagac ggagcaaatg atcgagaagg 121 aggcaggcaa ggagccggcg gagggcggcg gcggcgacgg ctcgcaccgc ctcggggacg 181 cccaggagat gcgcgcggtg gtgctggctg gcttcggggg gctcaacaag ctgcggctct 241 tcaggaaggc catgcccgag cctcaggacg gcgagctcaa gatccgcgtc aaagcctgtg 301 gattaaactt cattgacttg atggtgcgac aagggaatat tgacaaccct cccaagactc 361 ccctggtgcc aggatttgag tgttctggga ttgttgaagc tctgggggac agcgtgaaag 421 gatatgagat tggagaccgt gtcatggcat ttgtcaatta caatgcctgg gcagaggtgg 481 tctgcacacc agtggagttt gtctacaaga tcccggatga catgagcttc tcggaggctg 541 ctgcattccc catgaacttc gtcacagcct atgtgatgct gtttgaagtt gccaacctcc 601 gggaagggat gtctgtgctc gtgcactcag ctggtggggg cgtgggtcaa gctgtggctc 661 agctgtgttc cactgtcccc aacgtgactg tctttggaac agcctctact ttcaagcatg 721 aagcaatcaa agactctgtg acccacctct ttgacagaaa tgcagactac gtgcaagaag 781 ttaaaagaat ctctgctgaa ggtgtggaca tcgttttgga ttgcctctgt ggggacaaca 841 ctggaaaagg tctcagtctt ctcaaacccc tgggaaccTA CATTTTATAT GGCTCATCCA 901 ACATGGTAAC TGGAGAGACC AAGAGCTTCT TCAGCTTTGC AAAATCATGG TGGCAGGTGG 961 AGAAGGTGAA CCCCATCAAG CTGTATGAGG AGAACAAAGT CATCGCGGGG TTTTCCCTTT 1021 TAAATCTGCT CTTCAAACAA GGCCGGGCGG GCCTCATTCG GGGAGTGGTG GAAAAACTCA 1081 TAGGGCTCTA CAACCAGAAG AAGATCAAGC CTGTGGTGGA CTCCTTGTGG GCTCTGGAGG 1141 AGGTGAAGGA GGCCATGCAG CGGATTCACG ACCGAGGGAA CATTGGCAAG TTAATTCTGG 1201 ATGTAGAAAA GACCCCAACT CCACTGATGG CCAATGACAG CACAGAGACC AGTGAAGCAG 1261 GGGAAGAGGA GGAGGACCAC GAGGGAGACA GCGAGAACAA GGAGCGGATG CCCTTTATCC 1321 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGACG CTACACTAAT TACGTTCGAA CCACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt