Construct: ORF TRCN0000469537
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008071.1_s317c1
- Derived from:
- ccsbBroadEn_06808
- DNA Barcode:
- ATTGGACTTTTTTGATTCCCAGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMD13 (5719)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469537
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5719 | PSMD13 | proteasome 26S subunit, non... | NM_002817.4 | 99.6% | 99.7% | (many diffs) |
| 2 | human | 5719 | PSMD13 | proteasome 26S subunit, non... | NM_175932.3 | 92.1% | 88.4% | (many diffs) |
| 3 | human | 5719 | PSMD13 | proteasome 26S subunit, non... | XM_011520235.3 | 82% | 82.1% | (many diffs) |
| 4 | mouse | 23997 | Psmd13 | proteasome (prosome, macrop... | NM_011875.4 | 89.9% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggacgtaccg ggcttcctac agcagagcca gagctccggg cccgggcagc 121 ccgctgtgtg gcaccgtctg gaggagctct acacgaagaa gttgtggcat cagctgacac 181 ttcaggtgct tgattttgtg caggatccgt gctttgccca aggagatggt ctcattaagc 241 tttatgaaaa ctttatcagt gaatttgaac acagggtgaa tcctctgtcc ctcgtggaaa 301 tcattcttca cgtagttaga cagatgactg atcctaatgt ggctcttact tttctggaaa 361 agactcgtga gaaggtgaaa agtagtgatg aggcagtgat cctgtgtaaa acagcaattg 421 gagctctaaa attaaacatc ggggacctac aggttacaaa ggaaacaatt gaagatgttg 481 aagaaatgct caacaacctt cctggtgtga catcggttca cagtcgtttc tatgatctct 541 ccagtaaata ctatcaaaca atcggaaacc acgcgtccta ctacaaagat gctctgcggt 601 ttttgggctg tgttgacatc aaggatctac cagtgtctga gcagcaggag agagccttca 661 cgctggggct agcaggactt ctcggcgagg gagtttttaa ctttggagaa ctcctcatgc 721 accctgtgct ggagtccctg aggaatactg accggcagtg gctgattgac accctctatg 781 CCTTCAACAG TGGCAACGTA GAGCGGTTCC AGACTCTGAA GACTGCCTGG GGCCAGCAGC 841 CTGATTTAGC AGCTAATGAA GCCCAGCTTC TGAGGAAAAT TCAGTTGTTG TGCCTCATGG 901 AGATGACTTT CACACGACCT GCCAATCACA GACAACTCAC TTTTGAAGAA ATTGCCAAAA 961 GTGCTAAAAT CACAGTGAAT GAGGTGGAGC TTCTGGTGAT GAAGGCCCTT TCGGTGGGGC 1021 TGGTGAAAGG CAGTATAGAC GAGGTGGACA AACGAGTCCA CATGACCTGG GTGCAGCCCC 1081 GAGTGTTGGA TTTGCAACAG ATCAAGGGAA TGAAGGACCG CCTGGAGTTC TGGTGCACGG 1141 ATGTGAAGAG CATGGAGATG CTGGTGGAGC ACCAGGCCCA TGACATCCTC ACCTACCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAA TTGGACTTTT TTGATTCCCA GAGACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att