Construct: ORF TRCN0000469540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001773.1_s317c1
- Derived from:
- ccsbBroadEn_07751
- DNA Barcode:
- CACACAGACACCCCGAGGCTCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRR1 (11103)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469540
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11103 | KRR1 | KRR1 small subunit processo... | NM_007043.7 | 99.9% | 99.7% | 206A>G |
| 2 | mouse | 52705 | Krr1 | KRR1, small subunit (SSU) p... | NM_178610.4 | 85.4% | 91.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtctccctcg ctggagcggc cagaaaaagg cgctggaaaa agtgaatttc 121 gtaaccagaa gccgaagccg gagaaccaag atgaatcaga actccttacg gttcctgatg 181 gttggaagga accagctttt tccaaagagg acaatcccag aggacttttg gaggagagca 241 gtttcgcaac tttgttccca aaatacaggg gagcttactt gaaagagtgt tggccattgg 301 tgcagaaagc cttaaatgaa catcatgtta atgcaaccct ggacctgatc gaaggcagca 361 tgactgtttg tactacaaag aagacttttg atccatatat catcattagg gccagagatc 421 tgataaaact gttagcaagg agtgtttcat ttgaacaggc agtacgaatt cttcaggatg 481 atgttgcatg tgacatcatt aaaataggtt ctttagtaag gaataaagag agatttgtaa 541 aacgaagaca acggcttatt ggtcccaaag gatctacatt gaaggcattg gaactcttaa 601 ctaattgtta cattatggtt cagggaaaca cagtttcagc cattggacct tttagtggct 661 taaaagaggt tagaaaagta gtccttgata ctatgaagaa tattcatcca atttataaca 721 ttaaaagctt aatgattaag agagagttgg caaaagattc TGAATTACGA TCACAAAGTT 781 GGGAGAGATT TTTGCCACAG TTCAAACACA AAAATGTGAA TAAACGCAAG GAACCAAAGA 841 AAAAAACTGT TAAGAAAGAA TATACGCCAT TCCCACCACC ACAACCAGAA AGTCAGATCG 901 ATAAAGAATT GGCTAGTGGT GAATACTTTT TGAAGGCAAA TCAGAAGAAG CGGCAGAAAA 961 TGGAAGCAAT AAAGGCTAAA CAAGCAGAAG CCATCAGTAA GAGACAAGAG GAAAGAAACA 1021 AAGCATTTAT TCCACCTAAG GAAAAACCAA TTGTGAAACC TAAGGAAGCT TCTACTGAAA 1081 CTAAAATTGA TGTGGCCAGC ATCAAGGAAA AGGTTAAGAA AGCAAAGAAT AAGAAACTGG 1141 GAGCTCTTAC AGCTGAAGAA ATTGCACTTA AGATGGAGGC AGATGAAAAG AAAAAGAAGA 1201 AAAAAAAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACACACA GACACCCCGA GGCTCCTTAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt