Construct: ORF TRCN0000469545
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004699.1_s317c1
- Derived from:
- ccsbBroadEn_12813
- DNA Barcode:
- AAGACTCTAAACACAAACGATTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCGF5 (84333)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469545
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84333 | PCGF5 | polycomb group ring finger 5 | XM_011540272.2 | 19.1% | 15.6% | (many diffs) |
| 2 | human | 84333 | PCGF5 | polycomb group ring finger 5 | XM_017016777.2 | 19.1% | 15.6% | (many diffs) |
| 3 | human | 84333 | PCGF5 | polycomb group ring finger 5 | NM_001256549.1 | 17.6% | 14.4% | (many diffs) |
| 4 | human | 84333 | PCGF5 | polycomb group ring finger 5 | NM_001257101.1 | 17.6% | 14.4% | (many diffs) |
| 5 | human | 84333 | PCGF5 | polycomb group ring finger 5 | NM_032373.5 | 17.6% | 14.4% | (many diffs) |
| 6 | human | 84333 | PCGF5 | polycomb group ring finger 5 | XM_011540271.3 | 17.6% | 14.4% | (many diffs) |
| 7 | human | 84333 | PCGF5 | polycomb group ring finger 5 | XM_017016776.2 | 17.6% | 14.4% | (many diffs) |
| 8 | human | 84333 | PCGF5 | polycomb group ring finger 5 | NR_046435.1 | 14.5% | 1_253del;401_1009del | |
| 9 | human | 84333 | PCGF5 | polycomb group ring finger 5 | NR_046310.1 | 12.5% | 1_418del;566_1174del | |
| 10 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | NM_029508.3 | 18.2% | 15.6% | (many diffs) |
| 11 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | XM_006527433.3 | 18.2% | 15.6% | (many diffs) |
| 12 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | XM_011247395.2 | 18.2% | 15.6% | (many diffs) |
| 13 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | XM_006527431.3 | 16.8% | 14.4% | (many diffs) |
| 14 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | XM_011247391.2 | 16.8% | 14.4% | (many diffs) |
| 15 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | XM_011247392.2 | 16.8% | 14.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 313
- ORF end:
- 460
- ORF length:
- 147
- Sequence:
-
1 TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA 61 GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC 121 ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG 181 AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA 241 GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC 301 AAAAAAGTTG GTATGGCTAC CCAAAGGAAA CACTTGGTGA AAGATTTTAA TCCTTACATT 361 ACCTGCTATA TCTGTAAAGG GTATCTGATC AAGCCAACAA CAGTGACGGA ATGCCTCCAT 421 ACATCTGCAG AATCCTACTG GATGTCCACT TGGATGTCCT GCCCAACTTT CTTGTACAAA 481 GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA 541 TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG 601 GACGAAAGAC TCTAAACACA AACGATTTGA CGCGTTAAGT Cgacaatcaa cctctggatt 661 acaaaatttg tgaaagatt