Construct: ORF TRCN0000469554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007980.1_s317c1
- Derived from:
- ccsbBroadEn_12976
- DNA Barcode:
- CCACATGACCCCTGTAGCACACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBTD2 (92181)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92181 | UBTD2 | ubiquitin domain containing 2 | XM_017010022.1 | 100% | 100% | |
| 2 | human | 92181 | UBTD2 | ubiquitin domain containing 2 | XM_017010023.1 | 100% | 100% | |
| 3 | human | 92181 | UBTD2 | ubiquitin domain containing 2 | NM_152277.3 | 81.1% | 81.1% | 1_132del |
| 4 | mouse | 327900 | Ubtd2 | ubiquitin domain containing 2 | NM_173784.3 | 72.3% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 636
- ORF length:
- 570
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agatggacaa ctacgcagca agagggatga attttgggat acagcaccag 121 cttttgaagg ccggaaagag atttgggatg ccttGAAGGC TGCTGCACAT GCTTTTGAGA 181 GCAATGATCA TGAACTGGCA CAAGCAATCA TTGATGGTGC AAACATAACA TTACCACATG 241 GTGCACTTAC AGAGTGCTAC GATGAACTGG GGAACAGATA TCAGCTTCCA GTGTATTGCT 301 TGGCACCGCC AATCAACATG ATAGAGGAAA AGAGCGACAT AGAGACTCTG GATATTCCTG 361 AGCCACCACC CAATTCTGGA TATGAATGTC AGCTTCGTTT GCGCCTTTCC ACAGGCAAAG 421 ACCTCAAGCT TGTGGTTCGC AGCACAGACA CAGTATTCCA CATGAAGAGA CGGTTGCATG 481 CAGCAGAGGG AGTGGAACCA GGTAGTCAGC GGTGGTTTTT TTCTGGCAGA CCTCTCACTG 541 ACAAAATGAA GTTCGAAGAG CTGAAGATCC CAAAGGACTA TGTTGTACAG GTTATAGTGA 601 GCCAACCTGT GCAGAACCCA ACACCAGTGG AGAACTGCCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 ACCACATGAC CCCTGTAGCA CACTTACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt