Construct: ORF TRCN0000469555
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010454.1_s317c1
- Derived from:
- ccsbBroadEn_05288
- DNA Barcode:
- ATCGGCGATACGACGTTCCCAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FNDC5 (252995)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469555
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 252995 | FNDC5 | fibronectin type III domain... | NM_001171941.3 | 100% | 100% | |
| 2 | human | 252995 | FNDC5 | fibronectin type III domain... | NM_153756.3 | 59.7% | 59.6% | 1_225del;634A>G;636_636delAins49 |
| 3 | human | 252995 | FNDC5 | fibronectin type III domain... | NM_001171940.2 | 46.1% | 45.6% | (many diffs) |
| 4 | mouse | 384061 | Fndc5 | fibronectin type III domain... | NM_027402.4 | 54.3% | 60.4% | (many diffs) |
| 5 | mouse | 384061 | Fndc5 | fibronectin type III domain... | XM_006503212.4 | 46.8% | 51.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGCT GCGCTTCATC CAGGAGGTGA ACACCACCAC CCGCTCATGT GCCCTCTGGG 121 ACCTGGAGGA GGATACGGAG TACATAGTCC ACGTGCAGGC CATCTCCATT CAGGGCCAGA 181 GCCCAGCCAG CGAGCCTGTG CTCTTCAAGA CCCCGCGTGA GGCTGAGAAG ATGGCCTCCA 241 AGAACAAAGA TGAGGTAACC ATGAAAGAGA TGGGGAGGAA CCAACAGCTG CGGACAGGCG 301 AGGTGCTGAT CATCGTCGTG GTCCTGTTCA TGTGGGCAGG TGTCATTGCC CTCTTCTGCC 361 GCCAGTATGA CATCATCAAG GACAATGAAC CCAATAACAA CAAGGAAAAA ACCAAGAGTG 421 CATCAGAAAC CAGCACACCA GAGCACCAGG GCGGGGGGCT TCTCCGCAGC AAGGTGAGGG 481 CAAGACCTGG GCCTGGGTGG GCCACCCTGT GCCTCATGCT CTGGTACCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA ATCGGCGATA CGACGTTCCC AATCACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt