Transcript: Human NM_001171941.3

Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
FNDC5 (252995)
Length:
3022
CDS:
219..680

Additional Resources:

NCBI RefSeq record:
NM_001171941.3
NBCI Gene record:
FNDC5 (252995)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001171941.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000128147 CAAAGATGAGGTAACCATGAA pLKO.1 398 CDS 100% 4.950 6.930 N FNDC5 n/a
2 TRCN0000128199 CAAGGACAATGAACCCAATAA pLKO.1 530 CDS 100% 13.200 9.240 N FNDC5 n/a
3 TRCN0000130811 GAGGAGGATACGGAGTACATA pLKO.1 279 CDS 100% 5.625 3.938 N FNDC5 n/a
4 TRCN0000129719 CCAAGAACAAAGATGAGGTAA pLKO.1 391 CDS 100% 4.950 3.465 N FNDC5 n/a
5 TRCN0000127728 GATGGCCTCCAAGAACAAAGA pLKO.1 383 CDS 100% 4.950 3.465 N FNDC5 n/a
6 TRCN0000130680 CCTTCCTTCTTCTGTCCTGTT pLKO.1 1428 3UTR 100% 4.050 2.835 N FNDC5 n/a
7 TRCN0000130041 GAACAAAGATGAGGTAACCAT pLKO.1 395 CDS 100% 3.000 2.100 N FNDC5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001171941.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05288 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_05288 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000469555 ATCGGCGATACGACGTTCCCAATC pLX_317 98.7% 100% 100% V5 n/a
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