Construct: ORF TRCN0000469558
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011003.1_s317c1
- Derived from:
- ccsbBroadEn_11718
- DNA Barcode:
- CATGGACCTATTCTGCATCAAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA0930 (23313)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469558
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23313 | KIAA0930 | KIAA0930 | XM_011530017.2 | 100% | 100% | |
| 2 | human | 23313 | KIAA0930 | KIAA0930 | XM_017028672.1 | 95.8% | 95.8% | 1_48del |
| 3 | human | 23313 | KIAA0930 | KIAA0930 | NM_001009880.1 | 91.5% | 91.5% | 1_102del |
| 4 | human | 23313 | KIAA0930 | KIAA0930 | NM_015264.1 | 90.4% | 90.4% | 1_117del |
| 5 | mouse | 223739 | 5031439G07Rik | RIKEN cDNA 5031439G07 gene | NM_001033273.2 | 73.7% | 79.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1176
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ctccacctac ttcatggaga aatgggctcc ccggcaggac gacatgcttt 121 tctatgtgcg ccggaagctg gcgtactccg gcagcgaaag cggtgcagac gggaggaagg 181 cagctgagcc tgaggtggag gtggaggtgt accggcggga ctccaagaag ctgccaggcc 241 tgggagaccc tgacatcgac tgggaggaga gcgtctgcct gaatctcatc ctgcagaagc 301 tggactacat ggtgacctgt gcggtgtgca cacgtgctga cggcggggac attcacatcc 361 ataagaagaa atctcagcaa gtgttcgcgt cccccagtaa acaccccatg gacagcaagg 421 gggaggagtc caagatcagc taccccaaca tcttcttcat gattgacagc ttcgaggagg 481 tgttcagcga catgaccgta ggggaaggag agatggtctg tgtggagctg gtggctagtg 541 acaaaaccaa cacgttccag ggggtcatct ttcagggctc catccgctac gaggcgctca 601 agaaggtgta tgacaaccgg gtgagcgtgg ccgcccgcat ggcacagaag atgtcgtttg 661 gcttctacaa gtacagcaac atggagtttg tgcgcatgaa gggcccccag ggcaagggcc 721 acgccgagat ggcggTCAGC CGAGTGTCTA CAGGTGACAC ATCCCCCTGT GGGACTGAAG 781 AGGACTCCAG CCCAGCTTCG CCCATGCACG AGCGGGTGAC CTCCTTCAGC ACACCCCCCA 841 CCCCAGAACG GAACAACCGG CCTGCCTTCT TCTCCCCATC CCTCAAGAGG AAGGTGCCCC 901 GGAACCGGAT CGCTGAGATG AAGAAGTCGC ACTCGGCCAA CGACAGCGAG GAGTTCTTCC 961 GGGAGGACGA CGGTGGAGCC GATCTGCACA ATGCAACCAA CCTGCGGTCT CGGTCCCTGT 1021 CGGGCACAGG ACGGTCCCTG GTCGGGTCCT GGCTGAAGCT GAACAGAGCA GATGGAAACT 1081 TCCTTCTCTA TGCACACTTA ACCTACGTCA CGTTGCCGCT GCATCGGATT TTAACAGACA 1141 TCCTGGAAGT TCGGCAGAAG CCCATCCTGA TGACCTACCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 ACATGGACCT ATTCTGCATC AAGCGACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt