Construct: ORF TRCN0000469572
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011610.1_s317c1
- Derived from:
- ccsbBroadEn_02564
- DNA Barcode:
- ACTTATCTTTTAGTTCCCTCCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPIN1 (10927)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469572
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10927 | SPIN1 | spindlin 1 | NM_006717.3 | 100% | 100% | |
| 2 | mouse | 20729 | Spin1 | spindlin 1 | NM_001283028.1 | 93% | 99.2% | (many diffs) |
| 3 | mouse | 20729 | Spin1 | spindlin 1 | NM_001283029.1 | 93% | 99.2% | (many diffs) |
| 4 | mouse | 20729 | Spin1 | spindlin 1 | NM_146043.4 | 93% | 99.2% | (many diffs) |
| 5 | mouse | 20729 | Spin1 | spindlin 1 | XM_006516893.3 | 93% | 99.2% | (many diffs) |
| 6 | mouse | 20729 | Spin1 | spindlin 1 | XM_006516894.3 | 93% | 99.2% | (many diffs) |
| 7 | mouse | 20729 | Spin1 | spindlin 1 | XM_017315469.1 | 93% | 99.2% | (many diffs) |
| 8 | mouse | 20729 | Spin1 | spindlin 1 | NM_001283030.1 | 83.8% | 89.6% | (many diffs) |
| 9 | mouse | 20729 | Spin1 | spindlin 1 | NM_011462.3 | 83.2% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 852
- ORF length:
- 786
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaccccattc ggaaagacac ctggccagcg gtccagagct gatgcaggcc 121 atgctggagt atctgccaac atgatgaaga agaggacatc ccacaaaaaa catcggagca 181 gtgtgggtcc gagcaaacct gtttcccagc cccggcggaa catcgtaggc tgcaggattc 241 agcatgggtg gaaagagggg aatggccctg ttacccagtg gaaaggaacc gttctggacc 301 aggtgcctgt aaatccttct ttgtatctta taaaatacga tggatttgac tgtgtttatg 361 gactagaact taataaagat gaaagagttt ctgcgcttga agtcctccct gatagagttg 421 cgacatctcg aatCAGCGAT GCACACTTGG CAGACACAAT GATTGGCAAA GCAGTGGAAC 481 ATATGTTTGA GACAGAGGAT GGTTCTAAAG ATGAGTGGAG GGGAATGGTC TTAGCACGTG 541 CACCTGTCAT GAACACATGG TTTTACATTA CCTATGAGAA AGACCCTGTC TTGTACATGT 601 ACCAACTCTT AGATGATTAC AAAGAAGGCG ACCTTCGCAT TATGCCTGAT TCCAATGATT 661 CACCTCCAGC AGAAAGGGAA CCAGGAGAAG TTGTGGACAG CCTGGTAGGC AAACAAGTGG 721 AATATGCCAA AGAAGATGGC TCGAAAAGGA CTGGCATGGT CATTCATCAA GTAGAAGCCA 781 AGCCCTCCGT CTATTTCATC AAGTTTGATG ATGATTTCCA TATTTATGTC TACGATTTGG 841 TGAAAACATC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 901 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 961 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACT TATCTTTTAG TTCCCTCCAC 1021 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t