Transcript: Human NM_006717.3

Homo sapiens spindlin 1 (SPIN1), mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
SPIN1 (10927)
Length:
4459
CDS:
254..1042

Additional Resources:

NCBI RefSeq record:
NM_006717.3
NBCI Gene record:
SPIN1 (10927)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006717.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000141614 CCTCACCCTAACTCTCTTATT pLKO.1 1348 3UTR 100% 13.200 10.560 N SPIN1 n/a
2 TRCN0000141981 GCCACAATGCAAGCATAGTTT pLKO.1 1374 3UTR 100% 5.625 4.500 N SPIN1 n/a
3 TRCN0000142329 CCTGATTCCAATGATTCACCT pLKO.1 833 CDS 100% 2.640 2.112 N SPIN1 n/a
4 TRCN0000140931 CCCTCACCCTAACTCTCTTAT pLKO.1 1347 3UTR 100% 13.200 9.240 N SPIN1 n/a
5 TRCN0000142238 GAGACAGAGGATGGTTCTAAA pLKO.1 677 CDS 100% 13.200 9.240 N SPIN1 n/a
6 TRCN0000141794 GAAAGAGTTTCTGCGCTTGAA pLKO.1 569 CDS 100% 4.950 3.465 N SPIN1 n/a
7 TRCN0000140398 GCCAAAGAAGATGGCTCGAAA pLKO.1 914 CDS 100% 4.950 3.465 N SPIN1 n/a
8 TRCN0000140812 GCCCTCCGTCTATTTCATCAA pLKO.1 970 CDS 100% 4.950 3.465 N SPIN1 n/a
9 TRCN0000140254 GAAGAAGAGGACATCCCACAA pLKO.1 334 CDS 100% 4.050 2.835 N SPIN1 n/a
10 TRCN0000141191 CACTTGGCAGACACAATGATT pLKO.1 632 CDS 100% 5.625 3.375 N SPIN1 n/a
11 TRCN0000072091 GATGGATTTGACTGTGTTTAT pLKO.1 527 CDS 100% 13.200 6.600 Y Spin1 n/a
12 TRCN0000334319 GATGGATTTGACTGTGTTTAT pLKO_005 527 CDS 100% 13.200 6.600 Y Spin1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006717.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02564 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02564 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000469572 ACTTATCTTTTAGTTCCCTCCACA pLX_317 53.2% 100% 100% V5 n/a
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