Construct: ORF TRCN0000469573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002922.1_s317c1
- Derived from:
- ccsbBroadEn_01227
- DNA Barcode:
- TCTGTGGTAGCATGCATCGGGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PMM1 (5372)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469573
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5372 | PMM1 | phosphomannomutase 1 | NM_002676.3 | 100% | 100% | |
| 2 | human | 5372 | PMM1 | phosphomannomutase 1 | XM_011530230.2 | 90.6% | 85.9% | (many diffs) |
| 3 | human | 5372 | PMM1 | phosphomannomutase 1 | XM_011530231.3 | 73.2% | 64.5% | 85_86ins118;163_164ins92 |
| 4 | human | 5372 | PMM1 | phosphomannomutase 1 | XM_011530229.2 | 68.9% | 59.5% | 86_306del;502_503ins92 |
| 5 | human | 5372 | PMM1 | phosphomannomutase 1 | XM_005261638.4 | 62.9% | 50.7% | 0_1ins199;82_83ins92 |
| 6 | human | 5372 | PMM1 | phosphomannomutase 1 | XM_011530232.1 | 62.9% | 50.7% | 0_1ins199;82_83ins92 |
| 7 | human | 5372 | PMM1 | phosphomannomutase 1 | XM_024452251.1 | 56.1% | 56.1% | 0_1ins345 |
| 8 | mouse | 29858 | Pmm1 | phosphomannomutase 1 | NM_013872.4 | 90.4% | 95% | (many diffs) |
| 9 | mouse | 29858 | Pmm1 | phosphomannomutase 1 | NM_001282040.1 | 70.2% | 73.2% | (many diffs) |
| 10 | mouse | 29858 | Pmm1 | phosphomannomutase 1 | XR_875301.2 | 52.5% | (many diffs) | |
| 11 | mouse | 29858 | Pmm1 | phosphomannomutase 1 | NM_001282041.1 | 48.7% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 852
- ORF length:
- 786
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agtcaccgcc caggcagccc gcaggaagga gcgcgtcctc tgcctgtttg 121 acgtggacgg gaccctcacg ccggctcgcc agaaaattga ccctgaggtg gccgccttcc 181 tgcagaagct acgaagtaga gtgcagatcg gtgtggtggg cggctctgac tactgtaaga 241 tcgctgagca gctgggtgac ggggatgaag tcattgagaa gtttgattat gtgtttgccg 301 agaacgggac ggtgcagtat aagcacggac gactgctctc caagcagacc atccagaacc 361 acctggggga ggagctgctg caggacttga tcaacttctg ccTCAGCTAC ATGGCCCTGC 421 TCAGGCTGCC CAAGAAGCGT GGAACCTTCA TCGAGTTCCG GAATGGCATG CTGAACATCT 481 CGCCCATCGG CCGGAGCTGC ACCCTGGAGG AGAGGATCGA GTTCTCCGAA CTGGACAAGA 541 AAGAGAAGAT CCGGGAGAAG TTCGTGGAAG CCCTGAAAAC AGAGTTTGCT GGCAAAGGGC 601 TGAGGTTCTC TCGAGGAGGC ATGATCAGCT TTGACGTCTT CCCCGAGGGC TGGGACAAGC 661 GCTACTGCCT GGATAGCCTG GACCAGGACA GCTTCGACAC CATCCACTTC TTTGGGAACG 721 AGACTAGCCC TGGTGGGAAC GACTTTGAGA TCTTTGCCGA CCCCCGGACT GTTGGCCACA 781 GCGTGGTGTC TCCTCAGGAC ACGGTGCAGC GATGCCGGGA GATTTTCTTC CCAGAGACAG 841 CTCATGAGGC GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 901 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 961 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCT GTGGTAGCAT GCATCGGGCC 1021 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t