Construct: ORF TRCN0000469582
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018149.1_s317c1
- Derived from:
- ccsbBroadEn_03320
- DNA Barcode:
- ACCTGAGCACGTCTCGGAATGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHMP5 (51510)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469582
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51510 | CHMP5 | charged multivesicular body... | NM_016410.6 | 100% | 100% | |
| 2 | human | 51510 | CHMP5 | charged multivesicular body... | NM_001195536.1 | 77.7% | 75.7% | (many diffs) |
| 3 | mouse | 76959 | Chmp5 | charged multivesicular body... | NM_029814.1 | 87.8% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 723
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccgactcttc gggaaagcga aacccaaggc tccgccgccc agcctgactg 121 actgcattgg cacggtggac agtagagcag aatccattga caagaagatt tctcgattgg 181 atgctgagct agtgaagtat aaggatcaga tcaagaagat gagagagggt cctgcaaaga 241 atatggtcaa gcagaaagcc ttgcgagttt taaagcaaaa gaggatgtat gagcagcagc 301 gggacaatct tgcccaacag tcattcaaca tggaacaagc caattataCC ATCCAGTCTT 361 TGAAGGACAC CAAGACCACG GTTGATGCTA TGAAACTGGG AGTAAAGGAA ATGAAGAAGG 421 CATACAAGCA AGTGAAGATC GACCAGATTG AGGATTTACA AGACCAGCTA GAGGATATGA 481 TGGAAGATGC AAATGAAATC CAAGAAGCAC TGAGTCGCAG TTATGGCACC CCAGAACTGG 541 ATGAAGATGA TTTAGAAGCA GAGTTGGATG CACTAGGTGA TGAGCTTCTG GCTGATGAAG 601 ACAGTTCTTA TTTGGATGAG GCAGCATCTG CACCTGCAAT TCCAGAAGGT GTTCCCACTG 661 ATACAAAAAA CAAGGATGGA GTTCTGGTGG ATGAATTTGG ATTGCCACAG ATCCCTGCTT 721 CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 781 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 841 GGCTTTATAT ATCTTGTGGA AAGGACGAAC CTGAGCACGT CTCGGAATGA CGACGCGTTA 901 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt