Construct: ORF TRCN0000469591
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015668.3_s317c1
- Derived from:
- ccsbBroadEn_14829
- DNA Barcode:
- GACCAACTCTGTTTCGCACCTGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RP2 (6102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469591
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6102 | RP2 | RP2 activator of ARL3 GTPase | NM_006915.3 | 100% | 100% | |
| 2 | mouse | 19889 | Rp2 | retinitis pigmentosa 2 homolog | NM_001290643.1 | 86% | 88% | (many diffs) |
| 3 | mouse | 19889 | Rp2 | retinitis pigmentosa 2 homolog | NM_133669.5 | 86% | 88% | (many diffs) |
| 4 | mouse | 19889 | Rp2 | retinitis pigmentosa 2 homolog | NM_001290644.1 | 77.1% | 79.7% | (many diffs) |
| 5 | mouse | 19889 | Rp2 | retinitis pigmentosa 2 homolog | XM_017318439.1 | 77.1% | 79.7% | (many diffs) |
| 6 | mouse | 19889 | Rp2 | retinitis pigmentosa 2 homolog | NR_110967.1 | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcttcttc tccaagagac ggaaggctga caaggagtcg cggcccgaga 121 acgaggagga gcggccaaag cagtacagct gggatcagcg cgagaaggtt gatccaaaag 181 actacatgtt cagtggactg aaggatgaaa cagtaggtcg cttacctggg acggtagcag 241 gacaacagtt tctcattcaa gactgtgaga actgtaacat ctatattttt gatcactctg 301 ctacagttac cattgatgac tgtactaact gcataatttt tctgggaccc gtgaaaggca 361 gcgtgttttt ccggaattgc agagattgca agtgcacatt agcctgccaa caatttcgtg 421 tgcgagattg tagaaagctg gaagtctttt tgtgttgtgc cactcaaccc atcattgagt 481 cttcctcaaa tatcaaattt ggatgttttc aatggtacta tcctgaatta gctttccagt 541 tcaaagatgc agggctaagt atcttcaaca atacatggag taacattcat gactttacac 601 ctgtgtcagg agaactcaac tggagccttc ttccagaaga tgctgtggtt caggactatg 661 ttcctataCC TACTACCGAA GAGCTCAAAG CTGTTCGTGT TTCCACAGAA GCCAATAGAA 721 GCATTGTTCC AATATCCCGG GGTCAGAGAC AGAAGAGCAG CGATGAATCA TGCTTAGTGG 781 TATTATTTGC TGGTGATTAC ACTATTGCAA ATGCCAGAAA ACTAATTGAT GAGATGGTTG 841 GTAAAGGCTT TTTCCTAGTT CAGACAAAGG AAGTGTCCAT GAAAGCTGAG GATGCTCAAA 901 GGGTTTTTCG GGAAAAAGCA CCTGACTTCC TTCCTCTTCT GAACAAAGGT CCTGTTATTG 961 CCTTGGAGTT TAATGGGGAT GGTGCTGTAG AAGTATGTCA ACTTATTGTA AACGAGATAT 1021 TCAATGGGAC CAAGATGTTT GTATCTGAAA GCAAGGAGAC GGCATCTGGA GATGTAGACA 1081 GCTTCTACAA CTTTGCTGAT ATACAGATGG GAATATGCCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 AGACCAACTC TGTTTCGCAC CTGGGACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt