Construct: ORF TRCN0000469611
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010066.1_s317c1
- Derived from:
- ccsbBroadEn_11934
- DNA Barcode:
- TTGACTCCTTATGGGCAATCTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ISOC1 (51015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469611
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51015 | ISOC1 | isochorismatase domain cont... | NM_016048.2 | 78.8% | 78.8% | 1_189del |
| 2 | mouse | 66307 | Isoc1 | isochorismatase domain cont... | NM_025478.3 | 69.5% | 77.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 771
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ccgcaaattc gtggtgcagc tgttcgccga ggagtggggc cagtacgtgg 121 acttgcccaa gggcttcgcg gtgagcgagc gctgcaaggt gcgcctcgtg ccgttgcaga 181 tccagctcac taccctggga aatcttacac cttcaagcac tgtgtttttc tgctgtgata 241 tgcaggaaag gttcagacca gccatcaagt attttgggga tattattagc gtgggacaga 301 gattgttgca aggggcccgg attttaggaa ttcctgttat tgtaacagaa caatacccTA 361 AAGGTCTTGG GAGCACGGTT CAAGAAATTG ATTTAACAGG TGTAAAACTG GTACTTCCAA 421 AGACCAAGTT TTCAATGGTA TTACCAGAAG TAGAAGCGGC ATTAGCAGAG ATTCCCGGAG 481 TCAGGAGTGT TGTATTATTT GGAGTAGAAA CTCATGTGTG CATCCAACAA ACTGCCCTGG 541 AGCTAGTTGG CCGAGGAGTC GAGGTTCACA TTGTTGCTGA TGCCACCTCA TCAAGAAGCA 601 TGATGGACAG GATGTTTGCC CTCGAGCGTC TCGCTCGAAC CGGGATCATA GTGACCACGA 661 GTGAGGCTGT TCTGCTTCAG CTGGTAGCTG ATAAGGACCA TCCAAAATTC AAGGAAATTC 721 AGAATCTAAT TAAGGCGAGT GCTCCAGAGT CGGGTCTGCT TTCCAAAGTA TACCCAACTT 781 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 841 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 901 CTTGTGGAAA GGACGATTGA CTCCTTATGG GCAATCTTTC ACGCGTTAAG TCgacaatca 961 acctctggat tacaaaattt gtgaaagatt