Construct: ORF TRCN0000469618
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015793.1_s317c1
- Derived from:
- ccsbBroadEn_06133
- DNA Barcode:
- ACCTCCCCCTTAATATCGTTATAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOR1A (1861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469618
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1861 | TOR1A | torsin family 1 member A | NM_000113.3 | 99.8% | 99.6% | 775G>C |
| 2 | human | 1861 | TOR1A | torsin family 1 member A | XR_929731.3 | 44.7% | (many diffs) | |
| 3 | mouse | 30931 | Tor1a | torsin family 1, member A (... | NM_144884.2 | 85.7% | 90.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1062
- ORF length:
- 996
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctgggccgg gccgtgctgg gcctgctgct gctggcgccg tccgtggtgc 121 aggcggtgga gcccatcagc ctgggactgg ccctggccgg cgtcctcacc ggctacatct 181 acccgcgtct ctactgcctc ttcgccgagt gctgcgggca gaagcggagc cttagccggg 241 aggcactgca gaaggatctg gacgacaacc tctttggaca gcatcttgca aagaaaatca 301 tcttaaatgc cgtgtttggt ttcataaaca acccaaagcc caagaaacct ctcacgctct 361 ccctgcacgg gtggacaggc accggcaaaa atttcgtcag caagatcatc gcagagaata 421 tttacgaggg tggtctgaac agtgactatg tccacctgtt tgtggccaca ttgcactttc 481 cacatgcttc aaacatcacc ttgtacaagg atcagttaca gttgtggatt cgaggcaacg 541 tgagtgcctg tgcgaggtcc atcttcatat ttgatgaaat ggataagatg catgcaggcc 601 tcatagatgc catcaagcct ttcctcgact attatgacct ggtggatggg gtctcctacc 661 agaaagccat gttcatattt cTCAGCAATG CTGGAGCAGA AAGGATCACA GATGTGGCTT 721 TGGATTTCTG GAGGAGTGGA AAGCAGAGGG AAGACATCAA GCTCAAAGAC ATTGAACACG 781 CGTTGTCTGT GTCGGTTTTC AATAACAAGA ACAGTGGCTT CTGGCACAGC AGCTTAATTC 841 ACCGGAACCT CATTGATTAT TTTGTTCCCT TCCTCCCCCT GGAATACAAA CACCTAAAAA 901 TGTGTATCCG AGTGGAAATG CAGTCCCGAG GCTATGAAAT TGATGAAGAC ATTGTAAGCA 961 GAGTGGCTGA GGAGATGACA TTTTTCCCCA AAGAGGAGAG AGTTTTCTCA GATAAAGGCT 1021 GCAAAACGGT GTTCACCAAG TTAGATTATT ACTACGATGA TTGCCCAACT TTCTTGTACA 1081 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1141 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1201 AGGACGAACC TCCCCCTTAA TATCGTTATA GACGCGTTAA GTCgacaatc aacctctgga 1261 ttacaaaatt tgtgaaagat t