Construct: ORF TRCN0000469621
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014659.1_s317c1
- Derived from:
- ccsbBroadEn_10538
- DNA Barcode:
- CTCTTTTGCTCGAACTACCCCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FMO9P (116123)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469621
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116123 | FMO9P | flavin containing dimethyla... | NR_002925.2 | 34.3% | 1_329del;803A>G;809_1390delinsA | |
| 2 | mouse | 240894 | Fmo9 | flavin containing monooxyge... | NM_001347135.1 | 27.2% | 28.2% | (many diffs) |
| 3 | mouse | 240894 | Fmo9 | flavin containing monooxyge... | XM_006496853.1 | 24.8% | 25.8% | (many diffs) |
| 4 | mouse | 240894 | Fmo9 | flavin containing monooxyge... | NM_172844.2 | 24.6% | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tagtatctac aaatctgtga ccatcaatac ttccaaggag atgatgtgct 121 tcagtgactt ccctgtccct gatcattttc ccaactacat gcacaactcc aaactcatgg 181 actacttcgg gatgtatgcc acacactttg gccTCCTGAA TTACATTCGT TTTAAGACTG 241 AAGTGCAAAG TGTGAGGAAG CACCCAGATT TTTCTATCAA TGGACAATGG GATGTTGTTG 301 TGGAGACTGA AGAGAAACAA GAGACTTTGG TCTTTGATGG GGTCTTAGTT TGCAGTGGAC 361 ACCACACAGA TCCCTACTTA CCACTTCAGT CCTTCCCAGG CATTGAGAAA TTTGAAGGCT 421 GTTATTTCCA TAGTCGGGAA TACAAAAGTC CCGAGGACTT TTCAGGGAAA AGAATCATAG 481 TGATCGGCAT TGGAAATTCT GGAGTGGATA TTGCGGTGGA GCTCAGTCGT GTAGCAAAGC 541 AGATATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTCTTTTGC TCGAACTACC CCATTACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt