Construct: ORF TRCN0000469631
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006795.1_s317c1
- Derived from:
- ccsbBroadEn_08039
- DNA Barcode:
- TTGTACGGTATTTGGCTGAATTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF285 (26974)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469631
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26974 | ZNF285 | zinc finger protein 285 | NM_001291489.2 | 100% | 100% | |
| 2 | human | 26974 | ZNF285 | zinc finger protein 285 | NM_152354.6 | 100% | 100% | |
| 3 | human | 26974 | ZNF285 | zinc finger protein 285 | NM_001291488.2 | 98.8% | 98.8% | 13_33del |
| 4 | human | 646915 | ZNF806 | zinc finger protein 806 | NM_001304449.1 | 94.3% | 79.1% | (many diffs) |
| 5 | human | 26974 | ZNF285 | zinc finger protein 285 | NM_001291490.2 | 73.7% | 73.7% | 0_1ins465 |
| 6 | human | 26974 | ZNF285 | zinc finger protein 285 | NM_001291491.2 | 73.7% | 73.7% | 0_1ins465 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1839
- ORF length:
- 1770
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gattaagttc caggaaaggg tgacattcaa ggatgtggct gttgtcttca 121 ccaaggaaga gctggcacta ttggataaag cccagataaa cctgtaccaa gatgtgatgc 181 tggaaaactt caggaacctc atgttagtga gagacgggat taaaaacaac attttgaatc 241 ttcaggcaaa ggggttaagt tacctttcgc aagaagtgct tcattgctgg cagatttgga 301 aacaaaggat ccgggattta actgtgagtc aggattatat cgtgaacctt caagaagagt 361 gttccccaca tttagaagat gtttccctca gtgaagagtg ggcaggcatt tctcttcaga 421 tttctgaaaa tgaaaactat gtagtaaatg ccattatcaa aaatcaagat atcacagcat 481 ggcaaagcct gacacaggtt cttaccccag aatcgtggag gaaagccaac ataatgaccg 541 agccccagaa ctctcaggga agatataagg gaatttacat ggaagagaaa ttgtacagac 601 gtgctcagca tgatgacagc ctcagttgga cctcatgtga tcatcatgag tcccaagaat 661 gtaaaggaga ggaccctggt agacatccca gctgtgggaa aaacttgggt atgaaatcaa 721 cggttgaaaa acgtaatgcg gcccatgtat taccacagcc tttcccatgt aataactgtg 781 gggtggcctt tgcagatgat acagatcctc atgtccatca cagcactcac ctaggagaaa 841 aatcttataa atgtgaccag tatggaaaga actttagtca gagccaagat cttatcgttc 901 attgtaaaac tcactctggc aagactccct atgaattcca cgaatggcct atgggctgca 961 aacagagctc agaccttccc agatatcaga aagtctcctc aggagacaaa ccctacaaat 1021 gtaaagaatg tggcaagggc ttcaggcgca gctcttccct tcacaaccat catcgagtcc 1081 acacagggga gatgccctac aaatgcgatg aatgtgggaa agggtttgga tttaggtcac 1141 ttctttgtat tcatcaggga gtacacacag ggaaaaagcc ctataaatgt gaagagtgtg 1201 ggaagggctt tgatcagagc tccaaccttc ttgtccatca gagagtccac actggagaga 1261 agccctacaa atgcagtgag tgtggcaagt gctttagttc aagctccgtt cttcaagtcc 1321 actggaggtt tcacacaggg gagaaaccat ataggtgtgg tgagtgtgga aagggcttca 1381 gccaatgtac acaccTTCAC ATTCACCAGA GAGTCCACAC AGGGGAGAAA CCATACAAAT 1441 GCAATGTGTG TGGAAAGGAT TTTGCGTATA GCTCTGTTCT TCACACTCAT CAGAGAGTTC 1501 ACACTGGAGA AAAACCATAT AAATGTGAAG TGTGTGGAAA GTGCTTCAGT TACAGTTCAT 1561 ATTTTCACTT ACATCAAAGA GATCACATCA GAGAGAAACC ATATAAATGT GATGAGTGTG 1621 GTAAAGGCTT CAGCCGGAAT TCAGATCTTA ATGTTCACCT CAGAGTCCAC ACAGGAGAGA 1681 GGCCCTATAA GTGTAAGGCA TGTGGTAAGG GCTTCAGTCG TAATTCATAC CTCCTTGCCC 1741 ATCAGAGAGT GCATATAGAT GAGACACAGT ACACACACTG TGAGCGTGGA AAGGACCTTC 1801 TGACTCATCA AAGACTACAT GAGCAGAGAG AAACATTATT GCCAACTTTC TTGTACAAAG 1861 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1921 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1981 ACGATTGTAC GGTATTTGGC TGAATTATAC GCGTTAAGTC gacaatcaac ctctggatta 2041 caaaatttgt gaaagatt