Construct: ORF TRCN0000469638
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005289.1_s317c1
- Derived from:
- ccsbBroadEn_04510
- DNA Barcode:
- CAAAGCGTCCTGGAGATGAAGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFCAB12 (90288)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469638
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90288 | EFCAB12 | EF-hand calcium binding dom... | NM_207307.3 | 100% | 100% | |
| 2 | human | 90288 | EFCAB12 | EF-hand calcium binding dom... | XM_011513293.2 | 92.1% | 92.1% | (many diffs) |
| 3 | human | 90288 | EFCAB12 | EF-hand calcium binding dom... | XM_011513294.2 | 91.9% | 91.9% | (many diffs) |
| 4 | human | 90288 | EFCAB12 | EF-hand calcium binding dom... | XM_011513295.2 | 89.6% | 89.5% | (many diffs) |
| 5 | human | 90288 | EFCAB12 | EF-hand calcium binding dom... | XM_011513297.2 | 70.1% | 66.7% | (many diffs) |
| 6 | human | 90288 | EFCAB12 | EF-hand calcium binding dom... | XM_011513298.1 | 54.2% | 52.2% | (many diffs) |
| 7 | human | 90288 | EFCAB12 | EF-hand calcium binding dom... | XM_011513299.2 | 52.9% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1782
- ORF length:
- 1716
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgacgactat gaagcgtacc acagtctgtt cttgtcgctg ctcggactct 121 gcccgtctaa gactcccatc aatgaaaatg ctcccgtctt tgatcctgaa ccggtcattg 181 cccactgctt caagcagttc cagcagaagg acttccgcct gcctcagacc cgccggcgaa 241 tcatcatggt gcctcgcaag gaggatcaga caccccttaa tcctgcatcc caacctcagg 301 ctcccccaaa gcccatcccc agcttcaaag ttctggaagc tagagatatc caagagcagc 361 cagaggacag gaagacctgg ctgagccaga ggtcgaagct gcggcaggag ctagagtcct 421 ttggtgatgt aaagaggtgg ctggagaaca agcccagcat cacgccttca gaggccaagg 481 tcttacacat gatccacgag gagcagagtg cccagccaaa tgcctcccag gcaactacca 541 ggaccaccag gaagaaagcc cccaggctct cccggctgtc ccgccagatg gtgccccagc 601 tccagctgcc cgagccccct gccctgtcgg tcatgtactc ctacctgcat agccgcaaga 661 tcaagatcct ggagatattt cacaaggtgg gccagggtga gaaccagaga atcaccaggg 721 aggagttcat cgcggctgta aaggcagtcg gagtccctct gaagaaccaa gaggtggagg 781 atatagtgat ctacctcagc tctcttggga agcacaacac catcaccatg gatatcctgg 841 ccaataccta caagcagtgg tctatggctc agcaaaggag cagcctggcc actgcaaggg 901 agcattatat cttggccaag cacagagatt ccctgaaggg tccgctcaag aagcaggagg 961 tggattcagc cccacagctt cccaaagtgg acctactgac ggtgcctgca gtcgacacgc 1021 agatggagac gcggcccatg accctggagg agatggagga agtgggcaag cggtaccgcg 1081 agcggcagcg acagcacaag ctcacgatcc cctccatcca gtacacggag caatgtcacc 1141 tggtgcgctg tgggaatcgg cactttgatg agcactgcct cccgtccacc atccacgggg 1201 atatgaggga gctcattgac tcggcccgca ggcacaactt tctggtctac ctgcaatgct 1261 ggaagctctg taagtcctat ggcctcccgc tgacagagga catcctcatg aaagccttgc 1321 tgtacccagg agacaagatc attttccaga tggacaaagt gtgccccatc cggcagccgg 1381 gaggctacta cTCTGACTGG AAGGTCTTTT CTCCGAATCT GGCTCTGCTC CGGTCCCAGG 1441 GCCCTGGCAA GTCTAAGAGG ACTGACAAGA AAACGCCAAA GAAAAGCAAG AAAATGCGCT 1501 TTAAGGAGTT TGAGGAATTT ACCAGGAAGC TGAAGGTGAA GAGGTCCAGT GGTCTGCAGC 1561 AAACACACCC CAATTCCTTC TGGCCGGGTC ATCTTCTGGA TAAGCTGCAG CTCTACCTGC 1621 CCACTGTGGC CACAGACCGG AGCCTGGCGC TCTTCAGTTG TGTTCAACAC CAGCCCCATG 1681 TCTACCCAGC CACCTACCAC CCTGACCACT GGTGGCCCCT TAGGAACAAG AACTACATGA 1741 CCCACGCCCA TTATGATGCC GCCAAGGTGT ACTACATCAA CTACCCAACT TTCTTGTACA 1801 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1861 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1921 AGGACGACAA AGCGTCCTGG AGATGAAGCT CACGCGTTAA GTCgacaatc aacctctgga 1981 ttacaaaatt tgtgaaagat t