Transcript: Human XM_011513295.2

PREDICTED: Homo sapiens EF-hand calcium binding domain 12 (EFCAB12), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
EFCAB12 (90288)
Length:
4184
CDS:
2125..3912

Additional Resources:

NCBI RefSeq record:
XM_011513295.2
NBCI Gene record:
EFCAB12 (90288)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011513295.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434966 GGAGTTCATCGCGGCTGTAAA pLKO_005 2895 CDS 100% 13.200 18.480 N EFCAB12 n/a
2 TRCN0000130639 CCACTGCAAGGGAGCATTATA pLKO.1 3062 CDS 100% 15.000 10.500 N EFCAB12 n/a
3 TRCN0000413765 ACCCAGGAGACAAGATCATTT pLKO_005 3452 CDS 100% 13.200 9.240 N EFCAB12 n/a
4 TRCN0000421043 GCCACTGCAAGGGAGCATTAT pLKO_005 3061 CDS 100% 13.200 9.240 N EFCAB12 n/a
5 TRCN0000421361 TAAGGAGTTTGAGGAATTTAC pLKO_005 3630 CDS 100% 13.200 9.240 N EFCAB12 n/a
6 TRCN0000150144 GAGTGTCAAAGAGTCAAACTA pLKO.1 3993 3UTR 100% 5.625 3.938 N EFCAB12 n/a
7 TRCN0000129818 CCTTAGGAACAAGAACTACAT pLKO.1 3846 CDS 100% 4.950 3.465 N EFCAB12 n/a
8 TRCN0000128067 CAGACACAAGAGGAGTGTCAA pLKO.1 3981 3UTR 100% 0.495 0.347 N EFCAB12 n/a
9 TRCN0000149945 GAGGAGTGTCAAAGAGTCAAA pLKO.1 3990 3UTR 100% 4.950 2.970 N EFCAB12 n/a
10 TRCN0000147363 GAAATCCTTTCAAAGAGGGAT pLKO.1 4016 3UTR 100% 2.640 1.584 N EFCAB12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011513295.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04510 pDONR223 100% 89.6% 89.5% None (many diffs) n/a
2 ccsbBroad304_04510 pLX_304 0% 89.6% 89.5% V5 (many diffs) n/a
3 TRCN0000469638 CAAAGCGTCCTGGAGATGAAGCTC pLX_317 23% 89.6% 89.5% V5 (many diffs) n/a
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