Construct: ORF TRCN0000469656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004128.1_s317c1
- Derived from:
- BRDN0000391739
- DNA Barcode:
- ATCGATTTATTTTAGCAGAAACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRMP1 (1400)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469656
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1400 | CRMP1 | collapsin response mediator... | NM_001313.5 | 99.7% | 99.4% | 20_22delAGAinsTCG;1342_1343delAGinsTA |
| 2 | human | 1400 | CRMP1 | collapsin response mediator... | NM_001288662.1 | 98.5% | 97.5% | (many diffs) |
| 3 | human | 1400 | CRMP1 | collapsin response mediator... | NM_001288661.2 | 97.9% | 97.5% | (many diffs) |
| 4 | human | 1400 | CRMP1 | collapsin response mediator... | NM_001014809.3 | 82.7% | 81.4% | (many diffs) |
| 5 | mouse | 12933 | Crmp1 | collapsin response mediator... | NM_007765.4 | 88.8% | 96.1% | (many diffs) |
| 6 | mouse | 12933 | Crmp1 | collapsin response mediator... | NM_001136058.2 | 73.7% | 78.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1782
- ORF length:
- 1716
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gtaccagggc aagatcggca tcccgcacat cacgagtgac cgactcctca 121 tcaaaggtgg acggatcatc aacgatgacc aatcccttta tgctgacgtc tacctggagg 181 atggacttat caaacaaata ggagagaact taatcgttcc tggtggagtg aagaccattg 241 aagccaacgg gcggatggtt attcccggag gtattgatgt caacacgtac ctgcagaagc 301 cctcccaggg gatgactgcg gctgatgact tcttccaagg gaccagggcg gcactggtgg 361 gcgggaccac gatgatcatt gaccatgttg ttcctgaacc tgggtccagc ctactgacct 421 ctttcgagaa gtggcacgaa gcagctgaca ccaaatcctg ctgtgattac tccctccacg 481 tggacatcac aagctggtac gatggcgttc gggaggagct ggaggtgctg gtgcaggaca 541 aaggcgtcaa ttccttccaa gtctacatgg cctataagga tgtctaccaa atgtccgaca 601 gccagctcta tgaagccttt accttcctta agggcctggg agctgtgatc ttggtccatg 661 cagaaaatgg agatttgata gctcaggaac aaaagcggat cctggagatg ggcatcacgg 721 gtcccgaggg ccatgccctg agcagacctg aagagctgga ggccgaggcg gtgttccggg 781 ccatcaccat tgcgggccgg atcaactgcc ctgtgtacat caccaaggtc atgagcaaga 841 gtgcagccga catcatcgct ctggccagga agaaagggcc cctagttttt ggagagccca 901 ttgccgccag cctggggacc gatggcaccc attactggag caagaactgg gccaaggctg 961 cggcgttcgt gacttcccct cccctgagcc cggaccctac cacgcccgac tacttgacct 1021 ccctactggc ctgtggggac ttgcaggtca caggcagcgg ccactgtccc tacagcactg 1081 cccagaaggc ggtgggcaag gacaacttta ccctgatccc cgagggtgtc aacgggatag 1141 aggagcggat gacggtcgtc tgggacaagg cggtggctac tggcaaaatg gatgagaacc 1201 agtttgtcgc tgtcaccagc accaatgcag ccaagatctt taacctgtac ccaaggaaag 1261 ggcggattgc cgtgggctcg gatgccgacg tggtcatctg ggaccccgac aagttgaaga 1321 ccataacagc caaaagtcac aagtcggcgg tggagtacaa catcttcgag ggtatggagt 1381 gccacggctc cccactagtg gtcatctacc agggcaagat cgtctttGAA GACGGAAACA 1441 TCAACGTCAA CAAGGGCATG GGCCGCTTCA TTCCGCGGAA GGCGTTCCCG GAGCACCTGT 1501 ACCAGCGCGT CAAAATCAGG AATAAGGTTT TTGGATTGCA AGGGGTTTCC AGGGGCATGT 1561 ATGACGGTCC TGTGTACGAG GTACCAGCTA CACCCAAATA TGCAACTCCC GCTCCTTCAG 1621 CCAAATCTTC GCCTTCTAAA CACCAGCCCC CACCCATCAG AAACCTCCAC CAGTCCAACT 1681 TCAGCTTATC AGGTGCCCAG ATAGATGACA ACAATCCCAG GCGCACCGGC CACCGCATCG 1741 TGGCGCCCCC TGGTGGCCGC TCCAACATCA CCAGCCTCGG TTGCCCAACT TTCTTGTACA 1801 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1861 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1921 AGGACGAATC GATTTATTTT AGCAGAAACG TACGCGTTAA GTCgacaatc aacctctgga 1981 ttacaaaatt tgtgaaagat t