Construct: ORF TRCN0000469667
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004479.1_s317c1
- Derived from:
- ccsbBroadEn_12785
- DNA Barcode:
- CGATCACCGATGTAAGTTTATTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2IRD2 (84163)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469667
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84163 | GTF2IRD2 | GTF2I repeat domain contain... | NM_001281447.1 | 38.3% | 32.1% | (many diffs) |
| 2 | human | 389524 | GTF2IRD2B | GTF2I repeat domain contain... | NM_001368301.1 | 38.3% | 32.1% | (many diffs) |
| 3 | human | 389524 | GTF2IRD2B | GTF2I repeat domain contain... | NM_001003795.3 | 25.5% | 24.1% | (many diffs) |
| 4 | human | 84163 | GTF2IRD2 | GTF2I repeat domain contain... | NM_173537.4 | 25.5% | 24.1% | (many diffs) |
| 5 | human | 389524 | GTF2IRD2B | GTF2I repeat domain contain... | NM_001368302.1 | 21.8% | 20.6% | (many diffs) |
| 6 | human | 84163 | GTF2IRD2 | GTF2I repeat domain contain... | NM_001368300.1 | 21.8% | 20.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 834
- ORF length:
- 768
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccaggtagca gtgtccaccc tgcctgttga agaagagtcc tcctcagaga 121 ccaggatggt ggtgacattc ctcgtgtctg ccctcgaatc catgtgtaaa gaactggccg 181 agtccaaggc agaagtggcc tgcatcgcag tgtacgaaac agacgtgttt gtcgtcggaa 241 ccgagagagg atgcgctttt gttaatgcca ggacggattt tcagaaagat tttgcaaaat 301 actgcgttgc agagggactg tgtgaggtga aacctccctg ccctgtgaac gggatgcagg 361 tccactcggg cgaaacggaa atactcagga aggcagtgga ggactatttc tgcttttgtt 421 atggtaaagc cttagggaca acagtgatgg tgcctgttcc cTATGAGAAG ATGCTGCGAG 481 ACCAGTCGGC TGTGGTAGTG CAGGGGCTTC CGGAAGGCGT TGCCTTTCAA CACCCTGAGA 541 ATTACGACCT TGCAACCCTG AAATGGATTT TGGAGAACAA AGCAGGGATT TCATTCATCA 601 TAAATAGACC CTTCCTAGGA CCAGAGAGTC AGCTGGGTGG CCCTGGGATG GTAACAGATG 661 CGGAGAGATC CATAGTATCA CCAAGTGAAA GCTGCGGCCC CATCAATGTG AAAACTGAAC 721 CCATGGAAGA TTCTGGTGGG TACCAAGATG CTTTTAGAAT CAAGTATCGG CCAAGCGTGG 781 TAGCTCACGC CTGTAATCCC AGCAATTTGG GAGGCCGAGG CAGGCGGATC ACTTGCCCAA 841 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 901 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 961 TATCTTGTGG AAAGGACGAC GATCACCGAT GTAAGTTTAT TTCACGCGTT AAGTCgacaa 1021 tcaacctctg gattacaaaa tttgtgaaag att