Construct: ORF TRCN0000469673
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004512.1_s317c1
- Derived from:
- ccsbBroadEn_03265
- DNA Barcode:
- GTCCCACACCTGTTTACAAGTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BFAR (51283)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469673
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51283 | BFAR | bifunctional apoptosis regu... | NM_016561.3 | 100% | 100% | |
| 2 | human | 51283 | BFAR | bifunctional apoptosis regu... | NM_001330500.2 | 72.2% | 72.2% | 261_262ins375 |
| 3 | human | 51283 | BFAR | bifunctional apoptosis regu... | XM_005255350.2 | 71.5% | 64.4% | 0_1ins179;84_85ins205 |
| 4 | mouse | 67118 | Bfar | bifunctional apoptosis regu... | NM_025976.5 | 85.9% | 90% | (many diffs) |
| 5 | mouse | 67118 | Bfar | bifunctional apoptosis regu... | NM_001177552.1 | 61.7% | 65.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1416
- ORF length:
- 1350
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaacctcag aaaagctatg tgaacacaat ggaccttgag agagatgaac 121 ctctcaaaag caccggccct cagatttctg ttagtgaatt ttcttgccac tgctgctacg 181 acatcctggt taaccccacc accttgaact gtgggcacag cttctgccgt cactgccttg 241 ctttatggtg ggcatcttca aagaaaacag aatgtccaga atgcagagaa aaatgggaag 301 gtttccccaa agtcagtatt ctcctcaggg atgccattga aaagttattt cctgatgcca 361 ttagactgag atttgaagac attcagcaga ataatgacat agtccaaagt cttgcagcct 421 ttcagaaata tgggaatgat cagattcctt tagctcctaa cacaggccga gcgaatcagc 481 agatgggagg gggattcttt tccggtgtgc tcacagcttt aactggagtg gcagtggtcc 541 tgctcgtcta tcactggagc agcagggaat ctgaacacga cctcctggtc cacaaggctg 601 tggccaaatg gacggcggaa gaagttgtcc tctggctgga gcagctgggc ccttgggcat 661 ctctttacag ggaaaggttt ttatctgaac gagtaaatgg aaggttgctt ttaactttga 721 cagaggaaga attttccaag acgccctata ccatagaaaa cagcagccac aggagagcca 781 tcctcatgga gctagaacgt gtcaaagcat taggcgtgaa gcccccccag aatctctggg 841 aatataaggc tgtgaaccca ggcaggtccc tgttcctgct atacgccctc aagagctccc 901 ccaggctgag tctgctctac ctgtacctgt ttgactacac cgacaccttc ctacctttca 961 tccacaccat ctgccctctg caagaagaca gctctggGGA GGACATCGTC ACCAAGCTTC 1021 TGGATCTTAA GGAGCCTACG TGGAAGCAGT GGAGAGAGTT CCTGGTCAAA TACTCCTTCC 1081 TTCCATACCA GCTGATTGCT GAGTTTGCTT GGGACTGGTT GGAGGTCCAT TACTGGACAT 1141 CACGGTTTCT CATCATCAAT GCTATGTTAC TCTCAGTTCT GGAATTATTC TCCTTTTGGA 1201 GAATCTGGTC GAGAAGTGAA CTGAAGACCG TGCCTCAGAG GATGTGGAGC CATTTCTGGA 1261 AAGTATCAAC GCAGGGGCTT TTTGTGGCCA TGTTCTGGCC CCTCATCCCT CAGTTTGTTT 1321 GCAACTGTTT GTTTTACTGG GCCCTGTACT TTAACCCAAT TATTAACATT GATCTTGTGG 1381 TCAAGGAACT CCGGCGGCTG GAAACCCAGG TGTTGTGCCC AACTTTCTTG TACAAAGTGG 1441 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1501 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1561 AGTCCCACAC CTGTTTACAA GTGCGACGCG TTAAGTCgac aatcaacctc tggattacaa 1621 aatttgtgaa agatt