Construct: ORF TRCN0000469721
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012823.1_s317c1
- Derived from:
- ccsbBroadEn_12908
- DNA Barcode:
- CGGAATGTGGGCCCTCCCGAAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPIL4 (85313)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469721
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85313 | PPIL4 | peptidylprolyl isomerase li... | NM_139126.4 | 39% | 39% | 1_900del |
| 2 | mouse | 67418 | Ppil4 | peptidylprolyl isomerase (c... | NM_026141.3 | 34.8% | 36.1% | (many diffs) |
| 3 | mouse | 67418 | Ppil4 | peptidylprolyl isomerase (c... | XR_001779581.1 | 15.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga caatgtgctt atagatgaca gaagaataca tgtggatttt agccagtcgg 121 ttgcaaaggt taaatggaaa ggaaaaggtg ggaaatacac caagagtgat ttcaaggagt 181 atgaaaaaga acaggataaa ccacctaatt tggttctgaa agataaagta aagcccaaac 241 aggatacaaa atacgatctt atattagatg agcaggccga agactcaaaa tcaagtcact 301 cacacacaag taaaaaacac aagaagaaaa cccatcactg ttctgaagag aaagaagatg 361 aggactacat gccaatcaaa aatactaatc aggatatcta tagagaaatg gggtttggtc 421 actatgaaga agaagaaagc tgttgggaga aacaaaagag tgaaaagaga gaccgaactc 481 agaaccgaag tcgtagccga tctcgagaga gggatggcca ttatagtaat agtcataaat 541 caaaatacca aacagatctt tatgaaagag aaaggagtaA AAAGAGAGAC CGAAGCAGAA 601 GTCCAAAGAA GTCCAAAGAT AAAGAAAAAT CTAAGTATAG ATGCCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGACGG AATGTGGGCC CTCCCGAAAC TACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t