Construct: ORF TRCN0000469730
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007246.2_s317c1
- Derived from:
- ccsbBroadEn_15197
- DNA Barcode:
- TATCATGTCAACCCTCGCGGGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRSK1 (84446)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469730
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84446 | BRSK1 | BR serine/threonine kinase 1 | XM_011527395.2 | 78.3% | 78.4% | (many diffs) |
| 2 | human | 84446 | BRSK1 | BR serine/threonine kinase 1 | XM_005259327.3 | 68.5% | 68.6% | (many diffs) |
| 3 | human | 84446 | BRSK1 | BR serine/threonine kinase 1 | XM_024451739.1 | 67% | 67.1% | (many diffs) |
| 4 | human | 84446 | BRSK1 | BR serine/threonine kinase 1 | NM_032430.2 | 60.6% | 60.6% | (many diffs) |
| 5 | human | 84446 | BRSK1 | BR serine/threonine kinase 1 | XR_430213.4 | 42.6% | (many diffs) | |
| 6 | mouse | 381979 | Brsk1 | BR serine/threonine kinase 1 | XM_017322326.1 | 67.8% | 74.1% | (many diffs) |
| 7 | mouse | 381979 | Brsk1 | BR serine/threonine kinase 1 | NM_001003920.3 | 54.9% | 60% | (many diffs) |
| 8 | mouse | 381979 | Brsk1 | BR serine/threonine kinase 1 | XM_017322325.1 | 53% | 57.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1485
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gagcctgcca tccaacggag agctggaccc cgacgtccta gagagcatgg 121 catcactggg ctgcttcagg gaccgcgaga ggctgcatcg cgagctgcgc agtgaggagg 181 agaaccaaga aaagatgata tattatctgc ttttggatcg gaaggagcgg tatcccagct 241 gtgaggacca ggacctgcct ccccggaatg atgttgaccc cccccggaag cgtgtggatt 301 ctcccatgct gagtcgtcac gggaagcggc gaccagagcg gaagtccatg gaagtcctga 361 gcatcaccga tgccgggggt ggtggctccc ctgtacccac acgacgggcc ttggagatgg 421 cccagcacag ccagagatcc cgtagcgtca gtggagcctc cacgggtctg tcctccagcc 481 ctctaagcag cccaaggagt ccggtctttt ccttttcacc ggagccgggg gctggagatg 541 aggctcgagg cgggggctcc ccgacttcca aaacgcagac gctgccttct cggggcccca 601 ggggtggggg cgccggggag cagcccccgc cccccagtgc ccgctccaca cccctgcccg 661 gccccccagg ctccccgcgc tcctctggcg ggaccccctt gcactcgcct ctgcacacgc 721 cccgggccag tcccaccggg accccgggga caacaccacc ccccagcccc ggcggtggcg 781 tcgggggagc cgcctggagg agtcgtctca actccatccg caacagcttc ctgggctccc 841 ctcgctttca ccggcgcaag atgcaggtcc ctaccgctga ggagatgtcc agcttgacgc 901 cagagtcctc cccggagctg gcaaaacgct cctggttcgg gaacttcatc tccttggaca 961 aagaagaaca aatattcctc gtgctaaagg acaaacctct cagcagcatc aaagcagaca 1021 tcgtccatgc ctttctgtcg atccccagcc tgagtcacag tgtgctgtca cagaccagct 1081 tcagggccga gtacaaagcc agtggcggcc cctccgtctt ccaaaagccc gtccgcttcc 1141 aggtggacat cagctcctct gagggtccag agccctcccc gcgacgggac ggcagcggag 1201 gtggtggcat ctactccgtc accttcactc tcatctcggg tcccagccgt cggttcaagc 1261 gagtggtgga gaccatccag gcacagctcc TGAGCACTCA TGACCAGCCC TCCGTGCAGG 1321 CCCTGGCAGA CGAGAAGAAC GGGGCCCAGA CCCGGCCTGC TGGTGCCCCA CCCCGAAGCC 1381 TGCAGCCCCC ACCCGGCCGC CCAGACCCAG AGCTGAGCAG CTCTCCCCGC CGAGCCCCCC 1441 CCAAGGACAA GAAGCTCCTG GCCACCAACG GGACCCCTCT GCCCTGCCCA ACTTTCTTGT 1501 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1561 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1621 GAAAGGACGA TATCATGTCA ACCCTCGCGG GCAGACGCGT TAAGTCgaca atcaacctct 1681 ggattacaaa atttgtgaaa gatt